Universidad Complutense de Madrid
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Production of the biotechnologically relevant AFP from Aspergillus giganteus in the yeast Pichia pastoris



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López García, Belén y Moreno, Ana Beatriz y San Segundo , Blanca y De los Ríos , Vivian y Manning, James M. y Gavilanes, José G. y Martínez del Pozo, Álvaro (2010) Production of the biotechnologically relevant AFP from Aspergillus giganteus in the yeast Pichia pastoris. Protein Expression and Purification, 70 . pp. 206-210. ISSN 1096-0279

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The mould Aspergillus giganteus produces a basic, low molecular weight protein (AFP) showing in vitro and in vivo antifungal properties against important plant pathogens. AFP is secreted as an inactive precursor containing
an amino-terminal extension of six amino acids (If-AFP) which is later removed to produce the active protein. The molecular basis to explain this behavior and the features that determine the fungal specificity of this protein are not completely solved. In this work, the mature AFP (AFP*) and a version of AFP with an extended amino-terminal (proAFP) have been cloned and produced in the yeast Pichia pastoris. The two proteins have been purified to homogeneity
and characterized from structural and functional points of view. AFP* produced is practically indistinguishable from the natural fungal protein in terms of its
spectroscopic and antifungal properties while proAFP is mostly inactive under identical assay conditions. The availability of an active AFP protein produced in
P. pastoris will allow getting further insights into the mode of action and targeting specificity of AFP by using site-directed mutagenesis approaches.

Tipo de documento:Artículo
Palabras clave:Antifungal, Antimicrobial, Cysteine-rich, Peptide
Materias:Ciencias > Química > Biología molecular
Ciencias > Química > Bioquímica
Código ID:10175
Depositado:23 Feb 2010 08:27
Última Modificación:23 Feb 2010 08:27

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