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Fluorescent in situ hybridization and flow cytometry as tools to evaluate the treatments for the control of slime-forming enterobacteria in paper mills

Torres García, Claudia Esperanza and Gibello, Alicia and Nande, Mar and Martín, Margarita and Blanco Suárez, Ángeles (2008) Fluorescent in situ hybridization and flow cytometry as tools to evaluate the treatments for the control of slime-forming enterobacteria in paper mills. Applied Microbiology and Biotechnology, 78 (5). pp. 889-897. ISSN 0175-7598 (print version); 1432-0614 (electronic version)

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Abstract

Slime formation is a serious problem nowadays
in the paper industry. Some enterobacteria are associated
with the formation of slime deposits in paper and board
mills. Detection and characterization of slime forming
bacteria, belonging to the genus Enterobacter, Raoultella,
and Klebsiella have been achieved by fluorescence in situ
hybridization (FISH), using one probe based on the
enterobacterial repetitive intergenic consensus sequence
and other two rRNA targeted oligonucleotide probes. The
effects of three kinds of antimicrobiological products
(biocides, dispersants, and enzymes) on these enterobacterial cells were analyzed by flow cytometry (FC). Biocides Butrol 1009 and 1072 were the most effective microbiocides against all enterobacterial cells analyzed, reaching 90% of dead bacteria after 24 h. However, the enzymatic treatment (Buzyme) was not equally efficient on enterobacteria and its microbiocide capacity varied depending on the type of microorganism. FISH and FC were effective tools to detect important slime forming enterobacteria and to select specific treatments to control microbial problems in the paper industry.


Item Type:Article
Additional Information:

The original publication is available at www.springerlink.com

Uncontrolled Keywords:FISH, Flow cytometry, Papermaking, Slime control
Subjects:Sciences > Chemistry > Water
Sciences > Chemistry > Molecular biology
Sciences > Chemistry > Chemical engineering
Sciences > Chemistry > Biochemistry
ID Code:11899
Deposited On:25 Jan 2011 13:25
Last Modified:06 Feb 2014 09:13

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