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The ribonucleolytic activity of the ribotoxin α-sarcin is not essential for in vitro protein biosynthesis inhibition

Alvarez García, Elisa and Diago Navarro, Elizabeth and Herrero Galán, Elías and García Ortega, Lucía and López Villarejo, Juan and Olmo López, Nieves and Díaz Orejas, Ramón and Gavilanes Franco, José G. and Martinez del Pozo, Álvaro (2011) The ribonucleolytic activity of the ribotoxin α-sarcin is not essential for in vitro protein biosynthesis inhibition. BBA-Proteins and Proteomics, 1814 . pp. 1377-1382. ISSN 1570-9639

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Abstract

Fungal ribotoxins are toxic secreted ribonucleases that cleave a conserved single phosphodiester bond located
at the sarcin/ricin loop of the larger rRNA. This cleavage inactivates ribosomes leading to protein biosynthesis
inhibition and cell death. It has been proposed that interactions other than those found at the active site of
ribotoxins are needed to explain their exquisite specific activity. The study presented shows the ability of a
catalytically inactive α-sarcin mutant (H137Q) to bind eukaryotic ribosomes and interfere with in vitro
protein biosynthesis. The results obtained are compatible with previous observations that α-sarcin can
promote cell death by a mechanism that is independent of rRNA cleavage, expanding the potential set of
activities performed by this family of toxins.


Item Type:Article
Uncontrolled Keywords:Ribotoxin, Restrictocin, Ribosome, Protein-synthesis
Subjects:Sciences > Chemistry > Molecular biology
Sciences > Chemistry > Biochemistry
Medical sciences > Biology > Biotechnology
ID Code:13212
Deposited On:07 Sep 2011 07:29
Last Modified:20 Mar 2012 08:02

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