Biblioteca de la Universidad Complutense de Madrid

The ribonucleolytic activity of the ribotoxin α-sarcin is not essential for in vitro protein biosynthesis inhibition

Impacto



Alvarez García, Elisa y Diago Navarro, Elizabeth y Herrero Galán, Elías y García Ortega, Lucía y López Villarejo, Juan y Olmo López, Nieves y Díaz Orejas, Ramón y Gavilanes Franco, José G. y Martinez del Pozo, Álvaro (2011) The ribonucleolytic activity of the ribotoxin α-sarcin is not essential for in vitro protein biosynthesis inhibition. BBA-Proteins and Proteomics, 1814 . pp. 1377-1382. ISSN 1570-9639

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URL Oficial: http://www.elsevier.com/wps/find/journaldescription.cws_home/635707/description#description



Resumen

Fungal ribotoxins are toxic secreted ribonucleases that cleave a conserved single phosphodiester bond located
at the sarcin/ricin loop of the larger rRNA. This cleavage inactivates ribosomes leading to protein biosynthesis
inhibition and cell death. It has been proposed that interactions other than those found at the active site of
ribotoxins are needed to explain their exquisite specific activity. The study presented shows the ability of a
catalytically inactive α-sarcin mutant (H137Q) to bind eukaryotic ribosomes and interfere with in vitro
protein biosynthesis. The results obtained are compatible with previous observations that α-sarcin can
promote cell death by a mechanism that is independent of rRNA cleavage, expanding the potential set of
activities performed by this family of toxins.


Tipo de documento:Artículo
Palabras clave:Ribotoxin, Restrictocin, Ribosome, Protein-synthesis
Materias:Ciencias > Química > Biología molecular
Ciencias > Química > Bioquímica
Ciencias Biomédicas > Biología > Biotecnología
Código ID:13212
Depositado:07 Sep 2011 07:29
Última Modificación:20 Mar 2012 08:02

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