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Tumour Cell Lines HT-29 and FaDu Produce Proinflammatory Cytokines and Activate Neutrophils In Vitro: Possible Applications for Neutrophil-Based Antitumour Treatment

Brú Espino, Antonio and Souto Andres, Joan Carles and Alcolea, Sonia and Anton, Rosa and Remacha, Angel and Camacho, Mercedes and Soler, Marta and Bru, Isabel and Porres, Amelia and Vila, Luis Tumour Cell Lines HT-29 and FaDu Produce Proinflammatory Cytokines and Activate Neutrophils In Vitro: Possible Applications for Neutrophil-Based Antitumour Treatment. Mediators of Inflammation . ISSN 0962-9351

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Official URL: http://www.hindawi.com/journals/mi/2009/817498/

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Abstract

There is evidence that polymorphonuclear neutrophils (PMNs) can exert severe antineoplastic effects. Cross-talk between tumour cells and endothelial cells (ECs) is necessary for the accumulation of PMN around a tumour. This work reports the ability of two PMN-sensitive, human, permanent cell lines-colorectal adenocarcinoma (HT-29) and pharyngeal squamous-cell carcinoma (FaDu) cells-to act as inflammatory foci. PMNs were cytotoxic to both lines, the adhesion of the PMNs to the tumour cells being important in this effect. The tumour cells released appreciable amounts of IL-8 and GRO alpha, and induced the transmigration of PMN through human microvascular-EC monolayers. Conditioning media associated with both lines induced the adhesion of PMN and the surface expression of ICAM-1 in microvascular-EC. In addition, FaDu-conditioning-medium strongly induced the production of proinflammatory cytokines by microvascular-EC. These results support the idea that tumour cells might normally induce a potent acute inflammatory response, leading to their own destruction.


Item Type:Article
Uncontrolled Keywords:Acute inflammatory response; granulocyte-mediated cytotoxicity; colony-stimulating factor; human-melanoma cells; polymorphonuclear leukocytes; immune-response; nonoxidative mechanisms; endothelial-cells; cancer patients; gro-alpha; cell biology; Immunology
Subjects:Sciences > Mathematics > Mathematical analysis
ID Code:14832
Deposited On:18 Apr 2012 10:19
Last Modified:06 Feb 2014 10:10

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