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Position-dependent expression of GADD45 alpha in rat brain tumours

Brú Espino, Antonio and Fresno, Carlos del and Soares Schanoski, Alessandra and Albertos, Sonia and Brú, Isabel and Porres, Amelia and Rollan Landeras, Eduardo and Dopazo, Ana and Casero Díaz-Cano, David and Gomez Piña, Vanesa and Garcia, Lourdes and Arnalich, Francisco and Alvarez, Rebeca and Rodriguez-Rojas, Alexandro and Fuentes-Prior, Pablo and López-Collazo, Eduardo (2007) Position-dependent expression of GADD45 alpha in rat brain tumours. Medical Oncology, 24 (4). pp. 436-444. ISSN 1357-0560

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Abstract

Although the complex and multifactorial process of tumour growth has been extensively studied for decades, our understanding of the fundamental relationship between tumour growth dynamics and genetic expression profile remains incomplete. Recent studies of tumour dynamics indicate that gene expression in solid tumours would depend on the distance from the centre of the tumour. Since tumour proliferative activity is mainly localised to its external zone, and taking into account that generation and expansion of genetic mutations depend on the number of cell divisions, important differences in gene expression between central and peripheral sections of the same tumour are to be expected. Here, we have studied variations in the genetic expression profile between peripheral and internal samples of the same brain tumour. We have carried out microarray analysis of mRNA expression, and found a differential profile of genetic expression between the two cell subsets. In particular, one major nuclear protein that regulates cell responses to DNA-damaging and stress signals, GADD45 alpha, was expressed at much lower levels in the peripheral zone, as compared to tumour core samples. These differences in GADD45 alpha mRNA transcription levels have been confirmed by quantitative analysis via real time PCR, and protein levels of GADD45 alpha also exhibit the same pattern of differential expression. Our findings suggest that GADD45 alpha might play a major role in the regulation of brain tumour invasive potential.

Item Type:Article
Uncontrolled Keywords:kinase-activity; cell-cycle; neuroblastoma-cells; growth; arrest; p21; protein; gene; p21(waf1/cip1); inhibition; tumour growth; GADD45 alpha; p21
Subjects:Sciences > Mathematics > Mathematical analysis
ID Code:14861
Deposited On:18 Apr 2012 10:39
Last Modified:06 Feb 2014 10:10

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