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Global transcriptome analysis of Lactococcus garvieae strains in response to temperature

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Aguado Urda, Mónica y Gibello Prieto, Alicia y Blanco, M del Mar y Fernández Garayzábal, José F y López Alonso, Victoria y López Campos, Guillermo H (2013) Global transcriptome analysis of Lactococcus garvieae strains in response to temperature. PloS one, 8 (11). e79692. ISSN 1932-6203

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URL Oficial: http://dx.doi.org/10.1371/journal.pone.0079692



Resumen

Lactococcus garvieae is an important fish and an opportunistic human pathogen. The genomic sequences of several L. garvieae strains have been recently published, opening the possibility of global studies on the biology of this pathogen. In this study, a whole genome DNA microarray of two strains of L. garvieae was designed and validated. This DNA microarray was used to investigate the effects of growth temperature (18°C and 37°C) on the transcriptome of two clinical strains of L. garvieae that were isolated from fish (Lg8831) and from a human case of septicemia (Lg21881). The transcriptome profiles evidenced a strain-specific response to temperature, which was more evident at 18°C. Among the most significant findings, Lg8831 was found to up-regulate at 18°C several genes encoding different cold-shock and cold-induced proteins involved in an efficient adaptive response of this strain to low-temperature conditions. Another relevant result was the description, for the first time, of respiratory metabolism in L. garvieae, whose gene expression regulation was temperature-dependent in Lg21881. This study provides new insights about how environmental factors such as temperature can affect L. garvieae gene expression. These data could improve our understanding of the regulatory networks and adaptive biology of this important pathogen.


Tipo de documento:Artículo
Materias:Ciencias Biomédicas > Veterinaria
Código ID:39642
Depositado:21 Dic 2016 09:35
Última Modificación:21 Dic 2016 09:35

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