Universidad Complutense de Madrid
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Splitting of a prevalent Mycobacterium bovis spoligotype by variable-number tandem-repeat typing reveals high heterogeneity in an evolving clonal group

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Rodriguez Campos, Sabrina y Navarro, Yurena y Romero Martínez, Beatriz y Juan Ferré, Lucía de y Bezos Garrido, Javier y Mateos García, Ana y Golby, Paul y Smith, Noel H y Hewinson, Glyn R y Domínguez Rodríguez, Lucas y García de Viedma, Darío y Aranaz Martín, Alicia (2013) Splitting of a prevalent Mycobacterium bovis spoligotype by variable-number tandem-repeat typing reveals high heterogeneity in an evolving clonal group. Journal of clinical microbiology, 51 (11). pp. 3658-65. ISSN 1098-660X

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URL Oficial: http://dx.doi.org/10.1128 /JCM.01271-13



Resumen

Mycobacterium bovis populations in countries with persistent bovine tuberculosis usually show a prevalent spoligotype with a wide geographical distribution. This study applied mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) typing to a random panel of 115 M. bovis isolates that are representative of the most frequent spoligotype in the Iberian Peninsula, SB0121. VNTR typing targeted nine loci: ETR-A (alias VNTR2165), ETR-B (VNTR2461), ETR-D (MIRU4, VNTR580), ETR-E (MIRU31, VNTR3192), MIRU26 (VNTR2996), QUB11a (VNTR2163a), QUB11b (VNTR2163b), QUB26 (VNTR4052), and QUB3232 (VNTR3232). We found a high degree of diversity among the studied isolates (discriminatory index [D] = 0.9856), which were split into 65 different MIRU-VNTR types. An alternative short-format MIRU-VNTR typing targeting only the four loci with the highest variability values was found to offer an equivalent discriminatory index. Minimum spanning trees using the MIRU-VNTR data showed the hypothetical evolution of an apparent clonal group. MIRU-VNTR analysis was also applied to the isolates of 176 animals from 15 farms infected by M. bovis SB0121; in 10 farms, the analysis revealed the coexistence of two to five different MIRU types differing in one to six loci, which highlights the frequency of undetected heterogeneity.


Tipo de documento:Artículo
Materias:Ciencias Biomédicas > Veterinaria
Código ID:39646
Depositado:10 Ene 2017 08:50
Última Modificación:10 Ene 2017 08:50

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