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Rapid identification and differentiation of Mycobacterium avium subspecies paratuberculosis types by use of real-time PCR and high-resolution melt analysis of the MAP1506 locus

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Castellanos, Elena y Aranaz Martín, Alicia y De Buck, Jeroen (2010) Rapid identification and differentiation of Mycobacterium avium subspecies paratuberculosis types by use of real-time PCR and high-resolution melt analysis of the MAP1506 locus. Journal of clinical microbiology, 48 (4). pp. 1474-7. ISSN 1098-660X

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URL Oficial: http://jcm.asm.org/content/48/4/1474.full



Resumen

High-resolution melt (HRM) analysis can identify sequence polymorphisms by comparing the melting curves of amplicons generated by real-time PCR amplification. We describe the application of this technique to identify Mycobacterium avium subspecies paratuberculosis types I, II, and III. The HRM approach was based on type-specific nucleotide sequences in MAP1506, a member of the PPE (proline-proline-glutamic acid) gene family.


Tipo de documento:Artículo
Materias:Ciencias Biomédicas > Veterinaria
Código ID:39691
Depositado:10 Ene 2017 09:25
Última Modificación:10 Ene 2017 09:25

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