Universidad Complutense de Madrid
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Genetic diversity of Mycobacterium avium isolates recovered from clinical samples and from the environment: molecular characterization for diagnostic purposes

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Álvarez Sánchez, Julio y Gómez García, Ignacio y Aranaz Martín, Alicia y Bezos Garrido, Javier y Romero Martínez, Beatriz y Juan Ferré, Lucía de y Mateos García, Ana y Gómez Mampaso, Enrique y Domínguez Rodríguez, Lucas (2008) Genetic diversity of Mycobacterium avium isolates recovered from clinical samples and from the environment: molecular characterization for diagnostic purposes. Journal of clinical microbiology, 46 (4). pp. 1246-51. ISSN 1098-660X

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URL Oficial: http://jcm.asm.org/content/46/4/1246.full



Resumen

Isolation of Mycobacterium avium complex (MAC) organisms from clinical samples may occur in patients without clinical disease, making the interpretation of results difficult. The clinical relevance of MAC isolates from different types of clinical samples (n = 47) from 39 patients in different sections of a hospital was assessed by comparison with environmental isolates (n = 17) from the hospital. Various methods for identification and typing (commercial probes, phenotypic characteristics, PCR for detection of IS1245 and IS901, sequencing of the hsp65 gene, and pulsed-field gel electrophoresis) were evaluated. The same strain was found in all the environmental isolates, 21 out of 23 (91.3%) of the isolates cultured from urine samples, and 5 out of 19 (26.3%) isolates from respiratory specimens. This strain did not cause disease in the patients. Testing best characterized the strain as M. avium subsp. hominissuis, with the unusual feature that 81.4% of these isolates lacked the IS1245 element. Contamination of certain clinical samples with an environmental strain was the most likely event; therefore, characterization of the environmental mycobacteria present in health care facilities should be performed to discard false-positive isolations in nonsterile samples, mainly urine samples. Molecular techniques applied in this study demonstrated their usefulness for this purpose.


Tipo de documento:Artículo
Materias:Ciencias Biomédicas > Veterinaria
Código ID:39700
Depositado:10 Ene 2017 09:48
Última Modificación:10 Ene 2017 09:48

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