Universidad Complutense de Madrid
E-Prints Complutense

VIP impairs acquisition of the macrophage proinflammatory polarization profile

Impacto

Descargas

Último año

Carrión Caballo, Mar y Pérez García, Selene y Martínez, Carmen y Juarranz Moratilla, Yasmina y Estrada Capetillo, Lizbeth y Puig-Kröger, Amaya y Pérez Gomáriz, Rosa María y Gutiérrez Cañas, Irene (2016) VIP impairs acquisition of the macrophage proinflammatory polarization profile. Journal of leukocyte biology, 100 (6). pp. 1385-1393. ISSN 0741-5400

[img] PDF
Restringido a Sólo personal autorizado del repositorio

1MB

URL Oficial: http://www.jleukbio.org/content/100/6/1385.full.pdf+html



Resumen

This study tested the hypothesis that vasoactive intestinal peptide (VIP) is able to modify the macrophage inflammatory profile, thus supporting its therapeutic role in autoimmune diseases. Macrophages are innate immune cells that display a variety of functions and inflammatory profiles in response to the environment that critically controls their polarization.Deregulation between the pro- and anti-inflammatory phenotypes has been involved in different pathologies.Rheumatoid arthritis (RA) is an autoimmune disease, in which macrophages are considered central effectors of synovial inflammation, displaying a proinflammatory profile.VIP is a pleiotropic neuropeptide with proven antiinflammatory actions. As modulation of the macrophage phenotype has been implicated in the resolution of inflammatory diseases, we evaluated whether VIP is able to modulate human macrophage polarization. In vitropolarized macrophages by GM-CSF (GM-MØ), with a proinflammatory profile, expressed higher levels of VIP receptors, vasoactive intestinal polypeptide receptors 1 and 2 (VPAC1 and VPAC2, respectively), than macrophages polarized by M-CSF (M-MØ) with anti-inflammatory activities. RA synovial macrophages, according to their GM-CSF-like polarization state, expressed both VPAC1 and VPAC2. In vitro-generated GM-MØ exposed to VIP exhibited an up-regulation of M-MØ gene marker expression, whereas their proinflammatory cytokine profile was reduced in favor of an anti-inflammatory function. Likewise, in GM-MØ, generated in the presence of VIP, VIP somehow changes the macrophages physiology profile to a less-damaging phenotype. Therefore, these results add new value to VIP as an immunomodulatory agent on inflammatory diseases.


Tipo de documento:Artículo
Palabras clave:Granulocyte; Anti-inflammatory; Rheumatoid arthritis; Human monocyte; Inflammation
Materias:Ciencias Biomédicas > Medicina > Reumatología
Ciencias Biomédicas > Biología > Bioquímica
Código ID:41251
Depositado:10 Feb 2017 11:57
Última Modificación:16 May 2017 11:49

Descargas en el último año

Sólo personal del repositorio: página de control del artículo