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Tear cytokine profile of glaucoma patients treated with preservative-free or preserved latanoprost

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Martínez de la Casa, Jose Maria y Pérez Bartolomé, Francisco y Urcelay, Elena y Santiago, José Luis y Moreno Montañes, Javier y Arriola Villalobos, Pedro y Benítez del Castillo, José Manuel y García Feijoo, Julián (2017) Tear cytokine profile of glaucoma patients treated with preservative-free or preserved latanoprost. Ocular surface . ISSN 1542-0124 (En prensa)

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URL Oficial: https://doi.org/10.1016/j.jtos.2017.03.004




Resumen

Purpose: To determine variations in cytokine levels of glaucoma patients treated either with preservative-free latanoprost or preserved latanoprost, relative to healthy individuals. Methods: Tear samples were collected from 39 healthy subjects, 20 glaucoma patients treated with preserved latanoprost, and 20 patients treated with preservative-free latanoprost. A set of 27 inflammatory cytokines was analyzed in each group, including interleukin (IL)-1β, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL9, IL-10, IL-12 (p70), IL-13, IL-15, IL-17, eotaxin, fibroblast growth factor (FGF) basic, granulocyte colony stimulating factor (G-CSF), granulocyte monocyte colony stimulating factor (GM-CSF), interferon (IFN)-γ, interferon gamma-induced protein (IP)-10, monocyte chemo attractant protein (MCP)-1MCAF, macrophage inflammatory protein (MIP)-1α, MIP-1β, platelet-derived growth factor (PDGF)-BB, regulated on activation, normal T cell expressed and secreted (RANTES), tumor necrosis factor (TNF)-α and vascular endothelial growth factor (VEGF). Cytokine concentrations were obtained by the Bio-Plex Human Cytokine Immunoassay. Non-invasive tear breakup time (NI-TBUT), tear meniscus height, corneal fluorescein staining, conjunctival hyperemia and ocular surface disease index (OSDI) were assessed in patients treated with preservative-free and preserved latanoprost. Results: The levels of IL-2, IL-5, IL-10, IL-12 (p70), IL-13, IL-15, IL-17, FGF basic, PDGF-BB, and TNF-α were significantly higher in patients receiving preserved latanoprost, compared to normal controls (p < 0.05). The expression of all the cytokines studied remained statistically invariable in patients receiving preservative-free latanoprost, compared to healthy subjects (p > 0.05). Ocular surface parameters were not significantly different in both glaucoma groups, and no correlation between these clinical parameters and cytokine levels was observed. Conclusions: Treatment with preserved latanoprost has a direct impact on tear cytokine levels, whereas this effect is not observed upon preservative-free latanoprost instillation.


Tipo de documento:Artículo
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Received 14 December 2016, Revised 21 March 2017, Accepted 21 March 2017, Available online 22 March 2017.

Palabras clave:Cytokine; Glaucoma; Inflammation; Latanoprost; Ocular surface disease; Preservatives
Materias:Ciencias Biomédicas > Medicina > Oftalmología
Ciencias Biomédicas > Farmacia > Medicamentos
Código ID:42709
Depositado:02 Jun 2017 11:45
Última Modificación:22 Mar 2018 00:01

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