Biblioteca de la Universidad Complutense de Madrid

Peptide-induced negative selection of thymocytes activates transcription of an NF-kappa B inhibitor

Impacto



Fiorini, Emma y Reche, Pedro A (2002) Peptide-induced negative selection of thymocytes activates transcription of an NF-kappa B inhibitor.

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Resumen

Negative selection eliminates thymocytes bearing autoreactive T cell receptors (TCR) via an apoptotic mechanism. We have cloned an inhibitor of NF-kappa B, I kappa BNS, which is rapidly expressed upon TCR-triggered but not dexamethasone- or gamma irradiation-stimulated thymocyte death. The predicted protein contains seven ankyrin repeats and is homologous to I kappa B family members. In class I and class II MHC-restricted TCR transgenic mice, transcription of I kappa BNS is stimulated by peptides that trigger negative selection but not by those inducing positive selection (i.e., survival) or nonselecting peptides. I kappa BNS blocks transcription from NF-kappa B reporters, alters NF-kappa B electrophoretic mobility shifts, and interacts with NF-kappa B proteins in thymic nuclear lysates following TCR stimulation. Retroviral transduction of I kappa BNS in fetal thymic organ culture enhances TCR-triggered cell death consistent with its function in selection.


Tipo de documento:Artículo
Materias:Ciencias Biomédicas > Medicina > Inmunología
Ciencias Biomédicas > Biología > Biología molecular
Código ID:9342
Depositado:17 Aug 2009 08:49
Última Modificación:09 Oct 2014 08:57

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