Biblioteca de la Universidad Complutense de Madrid

Expression and characterization of the Trypanosoma cruzi dihydrofolate reductase domain

Impacto



Reche, Pedro A y Arrebola, R y Santi, D V y González-Pacanowska, D. y Ruiz Pérez, Luis Miguel (1995) Expression and characterization of the Trypanosoma cruzi dihydrofolate reductase domain. Molecular and Biochemical Parasitology, 76 (1-2). pp. 175-85. ISSN 0166-6851

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URL Oficial: http://www.elsevier.com/wps/find/journaldescription.cws_home/506086/description



Resumen

We have cloned and expressed in Escherichia coli a 702-base pair gene coding for the dihydrofolate reductase (DHFR) domain of the bifunctional dihydrofolate reductase-thymidylate synthase (DHFR-TS) from Trypanosoma cruzi. The DHFR domain was purified to homogeneity by methotrexate-Sepharose chromatography followed by an anion-exchange chromatography step in a mono Q column, and displayed a single 27-kDa band on SDS-PAGE. Gel filtration showed that the catalytic domain was expressed as a monomer. Kinetic parameters were similar to those reported for the wild-type bifunctional enzyme with Km values of 0.75 microM for dihydrofolate and 16 microM for NADPH and a kcat value of 16.5 s-1. T. cruzi DHFR is poorly inhibited by trimethoprim and pyrimethamine and the inhibition constants were always lower for the bifunctional enzyme. The binding of methotrexate was characteristic of a class of inhibitors that form an initial complex which isomerizes slowly to a tighter complex and are referred to as 'slow, tight-binding' inhibitors. While the slow-binding step of inhibition was apparently unaffected in the individually expressed DHFR domain, the overall inhibition constant was two-fold higher as a consequence of the superior inhibition constant value obtained for the initial inhibitory complex.


Tipo de documento:Artículo
Palabras clave:Trypanosoma cruzi; Dihydrofolate reductase; Protozoal enzyme; Heterologous expression; Folate metabolism
Materias:Ciencias Biomédicas > Biología > Bioquímica
Ciencias Biomédicas > Biología > Microbiología
Ciencias Biomédicas > Biología > Biología molecular
Código ID:9353
Depositado:14 Aug 2009 10:49
Última Modificación:09 Oct 2014 08:58

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