Universidad Complutense de Madrid
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Chromatin remodeling by the SWI/SNF complex is essential for transcription mediated by the yeast cell wall integrity MAPK pathway.

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Sanz, A. Belén y García, Raúl y Rodríguez-Peña, José Manuel y Díez-Muñiz, Sonia y Nombela, César y Peterson, Craig L. y Arroyo, Javier (2012) Chromatin remodeling by the SWI/SNF complex is essential for transcription mediated by the yeast cell wall integrity MAPK pathway. Molecular biology of the cell, 23 (14). pp. 2805-2817. ISSN 1939-4586

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URL Oficial: http://www.molbiolcell.org/content/23/14/2805



Resumen

In Saccharomyces cerevisiae, the transcriptional program triggered by cell wall stress is coordinated by Slt2/Mpk1, the mitogen-activated protein kinase (MAPK) of the cell wall integrity (CWI) pathway, and is mostly mediated by the transcription factor Rlm1. Here we show that the SWI/SNF chromatin-remodeling complex plays a critical role in orchestrating the transcriptional response regulated by Rlm1. swi/snf mutants show drastically reduced expression of cell wall stress-responsive genes and hypersensitivity to cell wall-interfering compounds. On stress, binding of RNA Pol II to the promoters of these genes depends on Rlm1, Slt2, and SWI/SNF. Rlm1 physically interacts with SWI/SNF to direct its association to target promoters. Finally, we observe nucleosome displacement at the CWI-responsive gene MLP1/KDX1, which relies on the SWI/SNF complex. Taken together, our results identify the SWI/SNF complex as a key element of the CWI MAPK pathway that mediates the chromatin remodeling necessary for adequate transcriptional response to cell wall stress.


Tipo de documento:Artículo
Palabras clave:Microbiología
Materias:Ciencias Biomédicas > Farmacia > Microbiología
Código ID:21895
Depositado:24 Jun 2013 13:38
Última Modificación:25 May 2015 10:23

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