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The transmembrane protein Opy2 mediates activation of the Cek1 MAP kinase in Candida albicans

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Herrero de Dios, Carmen María and Román García, Elvira and Diez, Carla and Alonso Monge, Rebeca and Pla Alonso, Jesús (2013) The transmembrane protein Opy2 mediates activation of the Cek1 MAP kinase in Candida albicans. Fungal genetics and biology : FG & B, 50 . pp. 21-32. ISSN 1096-0937

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Official URL: http://www.journals.elsevier.com/fungal-genetics-and-biology




Abstract

MAPK pathways are conserved and complex mechanisms of signaling in eukaryotic cells. These pathways mediate adaptation to different stress conditions by a core kinase cascade that perceives changes in the environment by different upstream elements and mediates adaptation through transcription factors. In the present work, the transmembrane protein Opy2 has been identified and functionally characterized in Candida albicans. This protein is required to trigger Cek1 phosphorylation by different stimuli such as the resumption of growth from stationary phase or the addition of the cell wall disturbing compounds zymolyase and tunicamycin. opy2 mutants display susceptibility to cell wall disturbing compounds like Congo red. However, it does not play a role in the adaptation to high osmolarity or oxidative stress, in close contrast with the situation for the homologous protein in Saccharomyces cerevisiae. The over-expression of Opy2 in a S. cerevisiae opy2ssk1 mutant partially complemented the osmosensitivity on solid medium by a Hog1-independent mechanism as well as the abnormal morphology observed in this mutant under high osmolarity. The electrophoretic pattern of CaOpy2 tagged version in S. cerevisiae suggested similar post-translational modification in both microorganisms. This protein is also involved in pathogenesis as revealed by the fact that opy2 mutants displayed a significantly reduced virulence in the Galleria mellonella model.


Item Type:Article
Uncontrolled Keywords:Microbiología
Palabras clave (otros idiomas):Microbiology
Subjects:Medical sciences > Biology > Microbiology
ID Code:21919
Deposited On:25 Jun 2015 12:41
Last Modified:11 Nov 2016 00:01

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