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Solving gap metabolites and blocked reactions in genome-scale models: application to the metabolic network of Blattabacterium cuenoti

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Ponce-de-Leon, Miguel and Montero, Francisco J and Peretó, Juli (2013) Solving gap metabolites and blocked reactions in genome-scale models: application to the metabolic network of Blattabacterium cuenoti. BMC Systems Biology, 7 (114). ISSN 1752-0509

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Official URL: http://www.biomedcentral.com/1752-0509/7/114



Abstract

Background:Metabolic reconstruction is the computational-based process that aims to elucidate the network of metabolites interconnected through reactions catalyzed by activities assigned to one or more genes. Reconstructed models may contain inconsistencies that appear as gap metabolites and blocked reactions. Although automatic methods for solving this problem have been previously developed, there are many situations where manual
curation is still needed.
Results:We introduce a general definition of gap metabolite that allows its detection in a straightforward manner.
Moreover, a method for the detection of Unconnected Modules, defined as isolated sets of blocked reactions
connected through gap metabolites, is proposed. The method has been successfully applied to the curation of
iCG238,the genome-scale metabolic model for the bacterium
Blattabacterium cuenoti, obligate endosymbiont of cockroaches.
Conclusion:We found the proposed approach to be a valuable tool for the curation of genome-scale metabolic
models. The outcome of its application to the genome-scale model B. cuenoti iCG238is a more accurate model ver-
sion named as B. cuenoti iMP240


Item Type:Article
Uncontrolled Keywords:Bacteria; genetics; metabolisms; symbiosis; biological model
Subjects:Medical sciences > Biology > Biomathematics
Medical sciences > Biology > Insects
Medical sciences > Biology > Microbiology
ID Code:29337
Deposited On:25 Mar 2015 11:59
Last Modified:26 Mar 2015 09:29

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