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1,3-Propanediol production by Klebsiella oxytoca NRRL-B199 from glycerol. Medium composition and operational conditions

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Wojtusik, Mateusz and Rodríguez Martín, Alberto and Ripoll Morales, Vanessa and Santos Mazorra, Victoria E. and García, Jose L. and García-Ochoa Soria, Félix (2015) 1,3-Propanediol production by Klebsiella oxytoca NRRL-B199 from glycerol. Medium composition and operational conditions. Biotechnology Reports, 6 . pp. 100-107. ISSN 8756-7938 (Print) 1520-6033 (Online)

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Official URL: http://www.sciencedirect.com/science/article/pii/S2215017X14000666



Abstract

Production of 1,3-propanediol from glycerol using Klebsiella oxytoca NRRL-B199 has been studied. Medium composition has been optimized by means of a statistical design based on the Taguchi method. Strong influences of glycerol and phosphate concentrations have been detected on biomass and product yields. Other factors, such as magnesium concentration and K:Na ratio, have shown a small influence on both responses, biomass and product concentrations. An optimized medium composition has been proposed, leading to a final 1,3-propanediol concentration of 12.4 g/L with a selectivity of 72% with respect to glycerol consumed at shaken bottle-scale. Once the medium composition had been optimized, the scale-up from shaken bottles to STBR was conducted. Several experiments in a 2 L STBR have been conducted in order to determine the best operating conditions concerning temperature and agitation. Under the best operating conditions, i.e., a programmed variable stirring rate ranging from 50 to 100 rpm and a temperature of 37 �C, a final concentration of 13.5 g/L of 1,3-propanediol with a selectivity of 86% with respect to the glycerol consumed was obtained.


Item Type:Article
Uncontrolled Keywords:1,3-Propanediol production, Klebsiella oxytoca, Medium composition, Operating conditions, Scale-up
Subjects:Sciences > Chemistry > Chemical engineering
ID Code:29624
Deposited On:16 Apr 2015 15:37
Last Modified:18 Jun 2015 08:26

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