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The effects of adiponectin and leptin on human endothelial cell proliferation: a live-cell study

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Álvarez, G. and Visitación Bartolomé, M. and Miana, M. and Jurado López, R. and Martín, R. and Zuluaga Arias, Pilar and Martínez Martínez, E. and Nieto, M.L. and Álvarez Sala, L.A. and Millán, J. and Lahera, V. and Cachofeiro, V. (2012) The effects of adiponectin and leptin on human endothelial cell proliferation: a live-cell study. Journal of vascular research, 49 (2). pp. 111-122. ISSN 1018-1172

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Abstract

The effect of adiponectin and leptin on the proliferation of the human microvascular endothelial cell line (HMEC-1) was studied in the absence or presence of fetal bovine serum (FBS). The participation of extracellular signal-regulated kinase (ERK) and phosphatidylinositol 3-kinase/Akt (PI-3K/Akt) pathways in this effect were evaluated. We studied the effect of both adipokines on the motility, mitosis, proliferation and cell death processes of HMEC-1 cells using live-cell imaging techniques. Adiponectin but not leptin further increased the proliferative effect induced by FBS on HMEC-1. This effect seems to be the consequence of an increase in the mitotic index in adiponectin-treated cells when compared to untreated ones. The presence of either the mitogen-activated protein kinase (MAPK) inhibitor (PD98059), or PI-3K inhibitor (LY294002), reduced the effect of adiponectin in a dose-dependent manner. Neither adipokine was able to affect HMEC-1 proliferation in FBS-free conditions. Duration of mitosis, cell motility and the cell death process were similar in all conditions. These data suggest that adiponectin and leptin exert different effects on endothelial cell function. Adiponectin was able to potentiate proliferation of HMEC-1. This effect involves the activation of both PI3-K/Akt and ERK/MAPK pathways. However, it seems to exert minimal effects on HMEC-1 function in the case of leptin


Item Type:Article
Uncontrolled Keywords:Adiponectin; Endothelial cells; Leptin; Live-cell image technique; Proliferation
Subjects:Sciences > Mathematics > Applied statistics
Medical sciences > Biology
ID Code:30526
Deposited On:29 May 2015 09:25
Last Modified:29 May 2015 09:25

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