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In vitro evaluation of graphene oxide nanosheets on immune function

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Feito Castellano, María José and Vila, Mercedes and Matesanz Sancho, Mª Concepción and Linares, J. and Gonçalves, G. and Marques, P.A.A.P. and Vallet Regí, María and Rojo, J.M. and Portolés Pérez, Teresa (2014) In vitro evaluation of graphene oxide nanosheets on immune function. Journal of Colloid and Interface Science (432). pp. 221-228. ISSN 0021-9797

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Official URL: http://dx.doi.org/10.1016/j.jcis.2014.07.004



Abstract

Graphene oxide (GO) has attracted the scientific community attention due to its novel properties and wide range of potential applications including hyperthermia cancer therapy. However, little is known about the GO effects on the immune function which involves both innate and adaptive defence mechanisms through the activation of different cell populations and secretion of several cytokines. The effect of different GO nanosheets designed for hyperthermia cancer therapy on macrophage and lymphocyte function should be determined before using GO for this application. Experiments The effects of GO nanosheets with 1 (1-GOs) and 6 arms (6-GOs) of polyethylene glycol on RAW-264.7 macrophages and primary splenocytes (as approximation to the in vivo situation) were evaluated through the proinflammatory cytokine secretion and the modulation of cell proliferation in the presence of specific stimuli for either T-lymphocytes (concanavalin A, anti-CD3 antibody) or B-lymphocytes/macrophages (lipopolysaccharide). Findings 6-GOs significantly increased the secretion of TNF-α by RAW-264.7 macrophages without alteration of IL-6 and IL-1β levels. The treatment of primary splenocytes with 1-GOs and 6-GOs in the presence of concanavalin A, anti-CD3 antibody and lipopolysaccharide, produced significant dose-dependent decreases of cell proliferation and IL-6 levels, revealing weak inflammatory properties of GOs which are favourable for hyperthermia cancer therapy.


Item Type:Article
Uncontrolled Keywords:Materiales biomédicos,
Palabras clave (otros idiomas):Biomedical Materials
Subjects:Sciences > Chemistry > Chemistry, Inorganic
ID Code:30897
Deposited On:29 Jun 2015 09:03
Last Modified:20 Jul 2015 08:21

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