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“Super p53” Mice Display Retinal Astroglial Changes

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Salazar Corral, Juan José and Gallego-Pinazo, Roberto and Hoz Montañana, María Rosa de and Pinazo-Durán, María Dolores and Rojas López, María Blanca and Ramírez Sebastián, Ana Isabel and Serrano Espinosa, Manuel and Ramirez Sebastian, Jose Manuel (2013) “Super p53” Mice Display Retinal Astroglial Changes. PLoS ONE, 8 (6). e65446. ISSN 1932-6203

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Official URL: http://dx.doi.org/10.1371/journal.pone.0065446




Abstract

Tumour-suppressor genes, such as the p53 gene, produce proteins that inhibit cell division under adverse conditions, as in the case of DNA damage, radiation, hypoxia, or oxidative stress (OS). The p53 gene can arrest proliferation and trigger death by apoptosis subsequent to several factors. In astrocytes, p53 promotes cell-cycle arrest and is involved in oxidative stress-mediated astrocyte cell death. Increasingly, astrocytic p53 is proving fundamental in orchestrating neurodegenerative disease pathogenesis. In terms of ocular disease, p53 may play a role in hypoxia due to ischaemia and may be involved in the retinal response to oxidative stress (OS). We studied the influence of the p53 gene in the structural and quantitative characteristics of astrocytes in the retina. Adult mice of the C57BL/6 strain (12 months old) were distributed into two groups: 1) mice with two extra copies of p53 (“super p53”; n = 6) and 2) wild-type p53 age-matched control, as the control group (WT; n = 6). Retinas from each group were immunohistochemically processed to locate the glial fibrillary acidic protein (GFAP). GFAP+ astrocytes were manually counted and the mean area occupied for one astrocyte was quantified. Retinal-astrocyte distribution followed established patterns; however, morphological changes were seen through the retinas in relation to p53 availability. The mean GFAP+ area occupied by one astrocyte in “super p53” eyes was significantly higher (p<0.05; Student’s t-test) than in the WT. In addition, astroglial density was significantly higher in the “super p53” retinas than in the WT ones, both in the whole-retina (p<0,01 Student’s t-test) and in the intermediate and peripheral concentric areas of the retina (p<0.05 Student’s t-test). This fact might improve the resistance of the retinal cells against OS and its downstream signalling pathways.


Item Type:Article
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© 2013 Salazar et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Uncontrolled Keywords:Astrocytes; Retina; Antioxidants; Tumor-supressor gene; Induced Cell-Death; Oxidative Stress; Rat retina; Macular Degeneration; Glial Cells, CDA-Damage; P53-Induced Apoptosis; Experimental Glaucoma; Antioxidant Function; Fluorescence microscopy
Subjects:Medical sciences > Medicine > Medical genetics
Medical sciences > Medicine > Neurosciences
Medical sciences > Medicine > Ophtalmology
Medical sciences > Optics > Eyes anatomy
ID Code:31161
Deposited On:01 Jul 2015 11:14
Last Modified:07 Jul 2015 11:43

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