Universidad Complutense de Madrid
E-Prints Complutense

The lack of upstream elements of the Cek1 and Hog1 mediated pathways leads to a synthetic lethal phenotype upon osmotic stress in Candida albicans

Impacto

Downloads

Downloads per month over past year

Herrero de Dios, Carmen and Alonso Monge, Rebeca and Pla Alonso, Jesús (2014) The lack of upstream elements of the Cek1 and Hog1 mediated pathways leads to a synthetic lethal phenotype upon osmotic stress in Candida albicans. Fungal Genetics and Biology, 69 . pp. 31-42. ISSN 1087-1845

[img] PDF
Restringido a Repository staff only

1MB

Official URL: http://dx.doi.org/10.1016/j.fgb.2014.05.010




Abstract

Different signal transduction pathways mediated by MAP kinases have been described in Candida albicans. These pathways sense different stimuli and, therefore, elaborate specific responses. Hog1 was identified as the MAPK that is primarily involved in stress response and virulence, while Cek1 was more specific to cell wall biogenesis, mating and biofilm formation. In the present work, mutants defective in both pathways have been characterized under osmotic stress. Both routes are required for a full response against high osmotic challenge, since mutants defective in both pathways displayed aberrant morphology, cell polarity defects and abnormal chitin deposition, which correlate with loss of viability and appearance of apoptotic markers. These alterations occurred in spite of proper Hog1 and Cek1 phosphorylation and increased intra-cellular glycerol accumulation. The relevance of both routes in virulence is shown as ssk1 msb2 sho1 opy2 mutants are avirulent in a mouse systemic model of infection and display reduced virulence in the Galleria mellonella model.


Item Type:Article
Uncontrolled Keywords:Cek1, HOG pathway, Osmotic stress, Cell wall integrity, Pathogenic fungus, MAPK, Candida albicans
Subjects:Medical sciences > Pharmacy > Microbiology
ID Code:31258
Deposited On:21 Jul 2015 10:06
Last Modified:21 Jul 2015 10:11

Origin of downloads

Repository Staff Only: item control page