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Antioxidant and Protective Mechanisms against Hypoxia and Hypoglycaemia in Cortical Neurons in Vitro

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Merino, José Joaquín and Roncero Romero, César and Oset Gasque, María Jesús and Naddaf, Ahmad and González Prieto, María Pilar (2014) Antioxidant and Protective Mechanisms against Hypoxia and Hypoglycaemia in Cortical Neurons in Vitro. International Journal of Molecular Sciences, 15 (2). p. 2475. ISSN 1422-0067

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Official URL: http://dx. doi.org/10.3390/ijms15022475




Abstract

In the present work, we have studied whether cell death could be induced in cortical neurons from rats subjected to different period of O2 deprivation and low glucose (ODLG). This “in vitro” model is designed to emulate the penumbra area under ischemia. In these conditions, cortical neurons displayed loss of mitochondrial respiratory ability however, nor necrosis neither apoptosis occurred despite ROS production. The absence of cellular death could be a consequence of increased antioxidant responses such as superoxide dismutase-1 (SOD1) and GPX3. In addition, the levels of reduced glutathione were augmented and HIF-1/3α overexpressed. After long periods of ODLG (12–24 h) cortical neurons showed cellular and mitochondrial membrane alterations and did not recuperate cellular viability during reperfusion. This could mean that therapies directed toward prevention of cellular and mitochondrial membrane imbalance or cell death through mechanisms other than necrosis or apoptosis, like authophagy, may be a way to prevent ODLG damage.


Item Type:Article
Uncontrolled Keywords:Cortical neurons, Cerebral ischemia,ROS formation, Antioxidant defenses, Mitochondrial membrane potential, Free radical, SOD1/caspase-3
Subjects:Medical sciences > Pharmacy > Biochemistry
ID Code:32463
Deposited On:27 Jul 2015 11:44
Last Modified:04 Aug 2015 10:18

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