Universidad Complutense de Madrid
E-Prints Complutense

The Nitric Oxide Donor SNAP-Induced Amino Acid Neurotransmitter Release in Cortical Neurons. Effects of Blockers of Voltage-Dependent Sodium and Calcium Channels

Impacto

Descargas

Último año

Merino, José Joaquín y Arce, Carmen y Naddaf, Ahmad y Bellver Landete, Víctor y González, María Pilar y Oset Gasque, María Jesús (2014) The Nitric Oxide Donor SNAP-Induced Amino Acid Neurotransmitter Release in Cortical Neurons. Effects of Blockers of Voltage-Dependent Sodium and Calcium Channels. Plos ONE, 9 (3). pp. 1-8. ISSN 1932-6203

[img]
Vista previa
PDF
662kB

URL Oficial: http://dx. doi.org/10.1371/journal.pone.0090703


URLTipo de URL
http://www.plosone.org/Editorial
https://doaj.org/SIN ESPECIFICAR


Resumen

Background: The discovery that nitric oxide (NO)functions as a signalling molecule in the nervous system has radically changed the concept of neuronal communication. NO induces the release of amino acid neurotransmitters but the underlying mechanisms remain to be elucidated.
Findings: The aim of this work was to study the effect of NO on amino acid neurotransmitter release (Asp, Glu, Gly and GABA) in cortical neurons as well as the mechanism underlying the release of these neurotransmitters. Cortical neurons were stimulated with SNAP, a NO donor, and the release of different amino acid neurotransmitters was measured by HPLC. The involvement of voltage dependent Na+ and Ca2+ channels as well as cGMP in its mechanism of action was evaluated.
Conclusions: Our results indicate that NO induces release of aspartate, glutamate, glycine and GABA in cortical neurons and that this release is inhibited by ODQ, an inhibitor of soluble guanylate cyclase. Thus, the NO effect on amino acid neurotransmission could be mediated by cGMP formation in cortical neurons. Our data also demonstrate that the Na+ and Ca2+ voltage- dependent calcium channels are involved in the NO effects on cortical neurons.


Tipo de documento:Artículo
Materias:Ciencias Biomédicas > Farmacia > Bioquímica
Código ID:32484
Depositado:29 Jul 2015 07:51
Última Modificación:29 Jul 2015 07:51

Descargas en el último año

Sólo personal del repositorio: página de control del artículo