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Strain typing of Zygosaccharomyces yeast species using a single molecular method based on polymorphism of the intergenic spacer region (IGS)

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Wrent, Petra and Rivas, Eva María and Martínez Peinado, José and Siloniz Jiménez, María Isabel de (2010) Strain typing of Zygosaccharomyces yeast species using a single molecular method based on polymorphism of the intergenic spacer region (IGS). International Journal of Food Microbiology, 142 (1-2). pp. 89-96. ISSN 0168-1605

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Official URL: http://www.sciencedirect.com/science/article/pii/S0168160510003338




Abstract

Unlike previously reported methods that need a combination of several typing techniques, we have 22
developed a single method for strain typing of the Zygosaccharomyces bailii, Z. mellis and Z. rouxii spoilage 23 species. Strains belonging to other species have also been included for comparison. We have demonstrated 24 that the IGS-PCR RFLP method has a high discriminative power. Considering the three endonucleases used in 25 this work, we have obtained a variability of 100% for Z. mellis and Z. rouxii strains and up to 70% for Z.bailii. 26 We have also detected two misidentified Z. mellis strains (CBS 711 and CBS 7412) which have RFLP patterns 27 with a set of bands characteristic of Z. rouxii strains. Sequencing of 26S rDNA D1/D2 domains and the 5.8-ITS 28 rDNA region confirmed these strains as Z. rouxii. The method also groups three certified hybrid strains of 29 Zygosaccharomyces in a separate cluster.


Item Type:Article
Uncontrolled Keywords:Yeast typing, Zygosaccharomyces rouxii, Z. mellis, Z.bailii, PCR-RFLP, IGS, rDNA, Food spoilage yeast
Subjects:Medical sciences > Biology > Biotechnology
Medical sciences > Biology > Microbiology
ID Code:32701
Deposited On:30 Jul 2015 10:59
Last Modified:10 Dec 2018 15:30

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