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Quantitative analysis of morphological changes in yeast colonies growing on solid medium: the eccentricity and Fourier indices

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Gil de Prado, Elena and Rivas, Eva María and Siloniz Jiménez, María Isabel de and Diezma, Belén and Barreiro, Pilar and Martínez Peinado, José (2014) Quantitative analysis of morphological changes in yeast colonies growing on solid medium: the eccentricity and Fourier indices. Yeast, 31 (11). pp. 431-440. ISSN 1097-0061

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Official URL: http://onlinelibrary.wiley.com/doi/10.1002/yea.3036/abstract




Abstract

The colony shape of four yeast species growing on agar medium was measured for 116 days by image analysis. Initially, all the colonies are circular, with regular edges. The loss of circularity can be quantitatively estimated by the eccentricity index, Ei, calculated as the ratio between their orthogonal vertical and horizontal diameters. Ei can increase from 1 (complete circularity) to a maximum of 1.17–1.30, depending on the species. One colony inhibits its neighbour only when it has reached a threshold area. Then, Ei of the inhibited colony increases proportionally to the area of the inhibitory colony. The initial distance between colonies affects those threshold values but not the proportionality, Ei /area; this inhibition affects the shape but not the total surface of the colony. The appearance of irregularities in the edges is associated, in all the species, not with age but with nutrient exhaustion. The edge irregularity can be quantified by the Fourier index, Fi , calculated by the minimum number of Fourier coefficients that are needed to describe the colony contour with 99% fitness. An ad hoc function has been developed in Matlab v. 7.0 to automate the computation of the Fourier coefficients. In young colonies, Fi has a value between 2 (circumference) and 3 (ellipse). These values are maintained in mature colonies of Debaryomyces, but can reach values up to 14 in Saccharomyces. All the species studied showed the inhibition of growth in facing colony edges, but only three species showed edge irregularities associated with substrate exhaustion.


Item Type:Article
Uncontrolled Keywords:Yeast colony shape; Inhibition among colonies; Colony eccentricity; Colony edge irregularity
Subjects:Medical sciences > Biology > Microbiology
ID Code:32714
Deposited On:30 Jul 2015 11:14
Last Modified:10 Dec 2018 15:30

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