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Immunologic responses to the major allergen of Olea Europaea in local and systemic allergic rhinitis subjects

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Campo, Paloma and Villalba Díaz, Mayte and Barrionuevo, Esther and Rondón, Carmen and Galindo, Luisa and Rodríguez, María José and López Rodríguez, Juan Carlos and Torres, María José and Blanca, Miguel and Mayorga, Cristobalina (2015) Immunologic responses to the major allergen of Olea Europaea in local and systemic allergic rhinitis subjects. Clinical and Translational Allergy, 5 (sup.4). ISSN 2045-7022

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Abstract

Evaluate: the in vivo and in vitro responses to nOle e 1 in allergic rhinitis (AR) and local allergic rhinitis (LAR) patients sensitized to olive tree pollen (OL) confirmed by nasal allergen provocation test (NAPT).
Methods: Twelve subjects with AR, 12 with LAR, and 12 subjects as control group (CG) were selected. Skin testing and NAPT with nOle e 1 were performed. ECP and tryptase were measured in nasal lavages before and after NAPT. Serum IgE to OL allergens were measured by ELISA. Basophil activation tests (BAT) with OL and nOle e 1 and dendritic cell maturation/proliferation studies were carried out. Results: All AR (12/12) and 10/12 (83%) of LAR had a +NAPT to nOle e 1. ECP levels in nasal lavages were significantly increased after NAPT in both AR and LAR compared with CG at 15 minutes (p<0.05). Serum IgE was positive only in AR. All AR had +BAT responses to OL and 10/12 to nOle e 1 (83%); 8/12 LAR (66.6%) had a +BAT with OL and 4/12 (33%) to nOle e 1, with only one subject of the control group with a +BAT to both OL and nOle e 1 (8%). Dendritic cell proliferation to nOle e 1 was increased in AR compared to LAR and CG (p=0.019 and p=0.001 respectively).Conclusion: Both AR and LAR had a similar in vivo response to nOle e 1 with release of inflammatory mediators. Specific basophil activation with OL and nOle e 1 was observed in LAR confirming previous data obtained with dust mites.


Item Type:Article
Uncontrolled Keywords:Basophil activation test, local allergic rhinitis, nasal allergen provocation test, purified allergen
Subjects:Medical sciences > Medicine > Allergy
Medical sciences > Biology > Biochemistry
ID Code:33117
Deposited On:09 Oct 2015 10:28
Last Modified:09 Oct 2015 11:05

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