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A Candida albicans PeptideAtlas

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Vialás, Vital and Sun, Zhi and Loureiro yPenha, Carla Verónica and Carrascal, Montserrat and Abián, J. and Monteoliva Díaz, Lucía and Deutsch, Eric W. and Aebersold, Ruedi and Moritz, Robert L. and Gil, Concha (2014) A Candida albicans PeptideAtlas. Journal of proteomics, 97 . pp. 62-68. ISSN 1874-3919 (In Press)

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Official URL: http://dx.doi.org/10.1016/j.jprot.2013.06.020




Abstract

Candida albicans public proteomic datasets, though growing steadily in the last few years, still have a very limited presence in online repositories. We report here the creation of a C. albicans PeptideAtlas comprising near 22,000 distinct peptides at a 0.24% False Discovery Rate (FDR) that account for over 2500 canonical proteins at a 1.2% FDR. Based on data from 16 experiments, we attained coverage of 41% of the C. albicans open reading frame sequences (ORFs) in the database used for the searches. This PeptideAtlas provides several useful features, including comprehensive protein and peptide-centered search capabilities and visualization tools that establish a solid basis for the study of basic biological mechanisms key to virulence and pathogenesis such as dimorphism, adherence, and apoptosis. Further, it is a valuable resource for the selection of candidate proteotypic peptides for targeted proteomic experiments via Selected Reaction Monitoring (SRM) or SWATH-MS.
BIOLOGICAL SIGNIFICANCE:

This C. albicans PeptideAtlas resolves the previous absence of fungal pathogens in the PeptideAtlas project. It represents the most extensive characterization of the proteome of this fungus that exists up to the current date, including evidence for uncharacterized ORFs. Through its web interface, PeptideAtlas supports the study of interesting proteins related to basic biological mechanisms key to virulence such as apoptosis, dimorphism and adherence. It also provides a valuable resource to select candidate proteotypic peptides for future (SRM) targeted proteomic experiments. This article is part of a Special Issue entitled: Trends in Microbial Proteomics.


Item Type:Article
Uncontrolled Keywords:Candida albicans, PeptideAtlas, Proteotypic peptides
Subjects:Medical sciences > Pharmacy > Microbiology
ID Code:33596
Deposited On:17 Nov 2015 09:08
Last Modified:07 Dec 2015 15:32

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