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Detailed characterization of Act d 12 and Act d 13 from kiwi seeds: implication in IgE cross-reactivity with peanut and tree nuts

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Sirvent, Sofía and Cantó, Belén and Gómez, Francisca and Blanca, Natalia and Cuesta-Herranz, Javier and Canto, Gabriela and Blanca, Miguel and Rodíguez, Rosalía and Villalba, Mayte and Palomares, Oscar (2014) Detailed characterization of Act d 12 and Act d 13 from kiwi seeds: implication in IgE cross-reactivity with peanut and tree nuts. Allergy, 69 (11). pp. 1481-1488. ISSN 1398-9995 (on line), 0105-4538 (print)

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Official URL: http://onlinelibrary.wiley.com/doi/10.1111/all.12486/full



Abstract

Background:Act d 12 (11S globulin) and Act d 13 (2S albumin) are two novel relevant allergens from kiwi seeds that might be useful to improve the diagnostic sensitivity and the management of kiwifruit-allergic patients.
Objective:To perform a comprehensive structural and immunological characterization of purified Act d 12 and Act d 13 from kiwi seeds.
Methods:Sera from 55 well-defined kiwifruit-allergic patients were used. Act d 12 and Act d 13 were purified by chromatographic procedures. Circular dichroism, mass spectrometry, concanavalin A detection, immunoblotting, enzyme-linked immunosorbent assays, basophil activation tests, and IgE-inhibition experiments were used.
Results:Act d 12 and Act d 13 were purified from kiwi seeds to homogeneity by combining size-exclusion, ion-exchange, and RP-HPLC chromatographies. Both purified allergens preserve the structural integrity and display typical features of their homologous counterparts from the 11S globulin and 2S albumin protein families, respectively. These allergens are released from kiwi seeds after oral and gastric digestion of whole kiwifruit, demonstrating their bioavailability after ingestion. The allergens retain the capacity to bind serum IgE from kiwifruit-allergic patients, induce IgE cross-linking in effector-circulating basophils, and display in vitro IgE cross-reactivity with homologous counterparts from peanut and tree nuts.
Conclusion:Purified Act d 12 and Act d 13 from kiwi seeds are well-defined molecules involved in in vitro IgE cross-reactivity with peanut and tree nuts. Their inclusion in component-resolved diagnosis of kiwifruit allergy might well contribute to improve the diagnostic sensitivity and the management of kiwifruit-allergic patients.


Item Type:Article
Uncontrolled Keywords:2S albumin Act d 13;11S globulin Act d 12;component-resolved diagnosis;kiwifruit allergy;peanut and tree nuts cross-reactivity
Subjects:Medical sciences > Medicine > Allergy
Medical sciences > Medicine > Biochemistry
ID Code:33606
Deposited On:15 Oct 2015 11:02
Last Modified:01 Dec 2015 00:01

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