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Membrane-perturbing properties of three peptides corresponding to the ectodomain of hepatitis C virus E2 envelope protein

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Pacheco, Beatriz y Gómez-Gutiérrez, Julián y Yélamos, Belén y Delgado, Carmen y Roncal, Fernando y Albar, Juan P. y Peterson, Darrell y Gavilanes, Francisco (2006) Membrane-perturbing properties of three peptides corresponding to the ectodomain of hepatitis C virus E2 envelope protein. Biochimica et Biophysica Acta - Biomembranes, 1758 (6). pp. 755-763. ISSN 0005-2736

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URL Oficial: http://www.sciencedirect.com/science/article/pii/S0005273606001714



Resumen

Based on the predicted capacity to interact with membranes at the interface, we have found three regions in the ectodomain of the hepatitis C virus envelope glycoprotein E2 (430-449, 543-560 and 603-624) with the ability to destabilize membranes. Three peptides corresponding to the sequence of these regions have been synthesized and their interaction with liposomes have been characterized. The three peptides were able to insert deeply into the hydrophobic core of negatively charged phospholipids as stated by fluorescence depolarization of the probe 1,6-diphenyl-1,3,5- hexatriene. Peptides E2430-449 and E2603-624 were able to induce aggregation of phosphatidylglycerol vesicles in a concentration-dependent manner both at neutral and acidic pH while peptide E2543-560 did not induce any increase of optical density at 360 nm in the concentration range studied. The three peptides induced lipid mixing and the release of the internal contents in a dose-dependent manner when acidic phospholipids were used. Fourier transformed infrared spectroscopy indicated that the peptides adopted mainly a β-sheet conformation which is not modified by the presence of acidic phospholipids. Taken together, our results point out to the involvement of these three regions in the fusion mechanism of HCV at the plasma membrane level.


Tipo de documento:Artículo
Palabras clave:TRANSFORM INFRARED-SPECTROSCOPY; 2 FUSION PEPTIDES; VIRAL FUSION; CELL-FUSION; GLYCOPROTEIN; MODEL; PH; DESTABILIZATION; SEQUENCES; RECEPTOR
Materias:Ciencias > Química > Bioquímica
Código ID:33614
Depositado:16 Oct 2015 08:04
Última Modificación:16 Oct 2015 08:04

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