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Interaction of preS domains of Hepatitis B Virus with phospholipid vesicles

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Núñez, Elena and Yélamos, Belén and Delgado, Carmen and Gómez-Gutiérrez, Julián and Peterson, Darrell L. and Gavilanes, Francisco (2009) Interaction of preS domains of Hepatitis B Virus with phospholipid vesicles. Biochimica et Biophysica Acta - Biomembranes, 1788 (2). pp. 417-424. ISSN 0005-2736

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Official URL: http://www.sciencedirect.com/science/article/pii/S0005273608003441



Abstract

The role of preS domains of the hepatitis B virus (HBV) envelope proteins in the first steps of viral infection has been restricted to their implication in virus attachment to a putative hepatocyte receptor. In order to explore a fusion activity in these regions, we used recombinant preS domains to characterize their interaction with liposomes. Binding experiments carried out with NBD-labeled proteins indicated that preS were able to interact in a monomeric way with acidic phospholipid vesicles, being the partition coefficient similar to that described for peptides which can insert deeply into bilayers. Fluorescence depolarization of DPH-labeled vesicles confirmed the specificity for negative charged phospholipids. Upon interaction the proteins induced aggregation, lipid mixing and release of internal contents of acidic vesicles at both acid and neutral pH in a concentration-dependent manner. Taken together, all these data indicate that preS domains are able to insert into the hydrophobic core of the bilayer. Moreover, the insertion resulted in a protein conformational change which increased the helical content. Therefore all these results suggest that, besides their participation in the recognition of a cellular receptor, the preS domains could be involved in the fusion mechanism of HBV with the plasma membrane of target cells.


Item Type:Article
Uncontrolled Keywords:HBV, preS domains, fusion, phospholipid membranes
Subjects:Sciences > Chemistry > Biochemistry
ID Code:33616
Deposited On:16 Oct 2015 08:04
Last Modified:16 Oct 2015 08:04

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