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Comparative proteomic study of Edwardsiella tarda strains with different degrees of virulence.

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Buján, Noemí and Hernández Haro, Carolina and Monteoliva Díaz, Lucía and Gil, Concha and Magariños, Beatriz (2015) Comparative proteomic study of Edwardsiella tarda strains with different degrees of virulence. Journal of proteomics, 127 (Part B). pp. 310-320. ISSN 1874-3919 (In Press)

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Official URL: http://dx.doi.org/10.1016/j.jprot.2015.05.008



Abstract

Edwardsiella tarda is an enteric opportunistic pathogen that causes a great loss in aquaculture. This species has been described as a phenotypical homogeneous group; in contrast, serological studies and molecular typing revealed a wide heterogeneity. In this work, a proteomic study of differential expression of a virulent isolate from turbot cultured in the Norwest of Spain in comparison with an avirulent collection strain was performed in order to recognize proteins involved in virulence. One hundred and three proteins that presented different abundance were successfully identified and classified into 11 functional categories according to their biological processes: amino acid, carbohydrate and lipid metabolism, tricarboxylic cycle, stress response and protein fate, protein synthesis, biogenesis of cellular components, cell rescue defence and virulence, cell membrane and transport, signal transduction and purine and pyrimidine metabolism. Twenty three protein spots detected only in turbot isolate were identified. It was shown that the same proteins appeared in different spots in the two isolates. Mass spectra obtained by MALDITOF/TOF of some of these proteins and DNA sequencing explained the changes as a result of different amino acid sequences. Several proteins related with the virulence of E. tarda (FliC, ArnA or FeSODI) were only detected in the turbot European isolate.


Item Type:Article
Uncontrolled Keywords:Edwardsiella tarda, DIGE, Comparative proteomics, Virulence, Fish disease
Subjects:Medical sciences > Pharmacy > Microbiology
ID Code:33651
Deposited On:26 Nov 2015 12:11
Last Modified:08 Sep 2016 23:01

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