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Global Proteomic Profiling of the Secretome of Candida albicans ecm33 Cell Wall Mutant Reveals the Involvement of Ecm33 in Sap2 Secretion.

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Gil Bona, Ana and Monteoliva Díaz, Lucía and Gil García, Concha (2015) Global Proteomic Profiling of the Secretome of Candida albicans ecm33 Cell Wall Mutant Reveals the Involvement of Ecm33 in Sap2 Secretion. Journal of proteome research, 14 (10). pp. 4270-81. ISSN 1535-3893

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Official URL: http://dx.doi.org/10.1021/acs.jproteome.5b00411



Abstract

Candida albicans secretes numerous proteins related to cell wall remodeling, adhesion, nutrient acquisition and host interactions. Also, extracellular vesicles containing cytoplasmic proteins are secreted into the medium. The C. albicans ecm33/ecm33 mutant (RML2U) presents an altered cell wall and is avirulent. The proteomic analysis of proteins secreted by RML2U cells identified a total of 170 proteins: 114 and 154 of which correspond to the vesicle-free secretome and extracellular vesicles, respectively. Notably, 98 proteins were common to both samples, and the groups most represented were metabolic and cell wall-related proteins. The results of this study showed that RML2U had an altered pattern of proteins secreted by the classical secretion pathway as well as the formation of extracellular vesicles, including their size, quantity, and protein composition. Specifically, the secretion of aspartic protease 2 (Sap2) was compromised but not its intracellular expression, with bovine serum albumin (BSA) degradation by RML2U being altered when BSA was used as the sole nitrogen source. Furthermore, as recent research links the expression of Sap2 to the TOR (Target Of Rapamycin) signaling pathway, the sensitivity of RML2U to rapamycin (the inhibitor of TOR kinase) was tested and found to be enhanced, connecting Ecm33 with this pathway.


Item Type:Article
Uncontrolled Keywords:Candida albicans, ECM33, extracellular vesicles, LC−MS/MS analysis, secreted proteins, secreted aspartyl protease (Sap2), rapamycin
Subjects:Medical sciences > Pharmacy > Microbiology
ID Code:33653
Deposited On:11 Apr 2016 12:05
Last Modified:20 Apr 2016 10:48

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