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High-yield production of a chimeric glycoprotein based on permuted E1 and E2 HCV envelope ectodomains

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Tello, Daniel and Rodríguez-Rodríguez, Mar and Yélamos, Belén and Gómez-Gutiérrez, Julián and Peterson, Darrell L. and Gavilanes, Francisco (2015) High-yield production of a chimeric glycoprotein based on permuted E1 and E2 HCV envelope ectodomains. Journal of Virological Methods, 213 . pp. 38-44. ISSN 0166-0934; 1879-0984

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Official URL: http://www.sciencedirect.com/science/article/pii/S0166093414004509



Abstract

In this report it is described for the first time the expression and purification of large quantities of a oluble and correctly folded chimeric recombinant protein, E2661E1340, containing the permuted Hepatitis C virus (HCV) glycoprotein ectodomains E1 (amino acids 192-340) and E2 (amino acids 384-661). Using the baculovirus/insect cell expression system, 8mg of secreted protein were purified from 1L of culture media, a yield 4 times higher than the described for its counterpart E1341E2661. This permuted chimeric protein is glycosylated and possesses a high tendency to self-associate. The fluorescence emission spectrum indicates that Trp residues occupy a relatively low hydrophobic environment. The secondary structure was determined by deconvolution of the far-UV circular dichroism spectrum yielding 13% α-helix structure, 49% extended structure and 38% non-ordered structure. E2661E1340 binds to antibodies present in human sera from HCV-positive patients, a binding that is blocked at different levels by a rabbit anti-E2661 antibody. All these structural and antigenic features of E2661E1340 are very similar to those described for E1340E2661, Thus, this high-yield isolated chimeric protein may be a valuable tool to study the first steps of the HCV infection.


Item Type:Article
Uncontrolled Keywords:Hepatitis C Virus, envelope protein, E1, E2, baculovirus, glycosylation
Subjects:Sciences > Chemistry > Biochemistry
ID Code:33660
Deposited On:16 Oct 2015 11:24
Last Modified:05 Dec 2015 00:01

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