Universidad Complutense de Madrid
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Chemoproteomic Approach to Explore the Target Profile of GPCR ligands: Application to 5-HT1A and 5-HT6 Receptors

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Gamo, Ana M. y Gónzalez-Vera, Juan A. y Rueda-Zubiaurre, Ainoa y Alonso, Dulce y Vázquez-Villa, Henar y Martín-Couce, Lidia y Palomares, Oscar y López, Juan A. y Martín-Fontecha, Mar y Benhamú, Bellinda y López-Rodríguez, María L. y Ortega-Gutiérrez, Silvia (2015) Chemoproteomic Approach to Explore the Target Profile of GPCR ligands: Application to 5-HT1A and 5-HT6 Receptors. Chemistry a European Journal, 21 . pp. 1-10. ISSN 1521-3765 (Online)

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URL Oficial: http://onlinelibrary.wiley.com/doi/10.1002/chem.201503101/full



Resumen

Determination of the targets of a compound remains an essential aspect in drug discovery. A complete understanding of all binding interactions is critical to recognize in advance both therapeutic effects and undesired consequences. However, the complete polypharmacology of many drugs currently in clinical development is still unknown, especially in the case of G-protein-coupled receptor (GPCR) ligands. In this work we have developed a chemoproteomic platform based on the use of chemical probes to explore the target profile of a compound in biological systems. As proof of concept, this methodology has been applied to selected ligands of the therapeutically relevant serotonin 5-HT1A and 5-HT6 receptors, and we have identified and validated some of their off-targets. This approach could be extended to other drugs of interest to study the targeted proteome in disease-relevant systems.


Tipo de documento:Artículo
Palabras clave:chemical probes · drug discovery · GPCRS · selectivity profiling · serotonin receptors
Materias:Ciencias > Química > Química orgánica
Código ID:34496
Depositado:26 Nov 2015 12:01
Última Modificación:29 Nov 2015 15:07

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