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The deletion of residues 268-292 of E1 impairs the ability of HCV envelope proteins to induce pore formation

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Lombana, Laura and Ortega Atienza, Sara and Gómez Gutierrez, Julián and Yélamos, Belén and Peterson, Darrell and Gavilanes, Francisco (2016) The deletion of residues 268-292 of E1 impairs the ability of HCV envelope proteins to induce pore formation. Virus Research . ISSN 1872-7492 (Online), 0168-1702 (print)

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Official URL: http://www.sciencedirect.com/science/article/pii/S0168170215301660



Abstract

We have obtained a chimeric protein containing the ectodomains of hepatitis C virus (HCV) envelope proteins but lacking the region 268-292 of E1. All its structural properties are coincident with those of the corresponding full length chimera. The deleted and entire chimeras were compared in terms of their membrane destabilizing properties. No differences were found in their ability to induce vesicle aggregation and lipid mixing but the deleted chimera showed a reduced capacity to promote leakage. The role of the deletion was also studied by obtaining HCV pseudoparticles (HCVpp). Both E1 and E2, and also the E1 deleted mutant, were incorporated into HCVpp to a similar level. However, HCVpp containing the E1 deleted protein are almost unable to infect Huh7 cells. These results point to the involvement of the region 268-292 in the formation of pores in the membrane necessary for the complete fusion of the membranes.


Item Type:Article
Uncontrolled Keywords:hepatitis C virus; viral envelope proteins; lipid-protein interaction;membrane fusion; protein spectroscopic properties.
Subjects:Medical sciences > Biology > Molecular biology
Medical sciences > Biology > Biochemistry
ID Code:36213
Deposited On:08 Mar 2016 12:16
Last Modified:06 Mar 2017 00:01

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