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Immunocytochemical study on the triple origin of the sphincter iris in the chick embryo

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Barrio Asensio, María del Carmen and Murillo González, Jorge Alfonso and Peña Melián, Ángel and Puerta Fonollá, Javier (1999) Immunocytochemical study on the triple origin of the sphincter iris in the chick embryo. Development Genes and Evolution, 209 (10). pp. 620-624. ISSN 0949-944X

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Official URL: http://dx.doi.org/10.1007/s004270050296




Abstract

The ontogenic development of the sphincter iris has been studied by immunocytochemistry and standard staining on chick embryos from stage 25 HH to the time of hatching. We have used the monoclonal antibody 13F4, a highly specific marker of muscular cells. We have observed three different regions in the iris. Tn the pupillary region, immunoreactive cells are in continuous contact with the inner epithelium of the pupillary margin. In the intermediate region, the outer epithelium forms buds of pigmented cells that emigrate toward the stroma. In this epithelium cells that are totally or partially unpigmented exist, and they are 13F4 positive. In the sphincter we have observed 13F4 positive cells with melanin granules. In the ciliary region, the immunoreactivity appears in dispersed mesenchymal cells. The present findings are consistent with a triple origin of the sphincter iris in the chick embryo. This muscle is derived from the inner epithelium of the pupillary margin, the intermediate region of the outer epithelium, and from the mesenchymal cells. The cells of the inner epithelium of the pupillary margin are differentiated into smooth muscle cells, and the remaining cells form striated muscle cells.


Item Type:Article
Additional Information:

Received: 17 March 1999 / Accepted: 17 May 1999

Uncontrolled Keywords:sphincter iris; chick embryo; iris development; monoclonal antibody 13F4
Subjects:Medical sciences > Medicine > Anatomy
Medical sciences > Medicine > Ophtalmology
ID Code:38523
Deposited On:15 Jul 2016 07:20
Last Modified:15 Jul 2016 07:20

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