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Epidermal Growth Factor Impairs Palatal Shelf Adhesion and Fusion in the T gf-β3 Null Mutant

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Barrio Asensio, María del Carmen and Río Sevilla, Aurora, del and Murillo González, Jorge Alfonso and Maldonado Bautista, Estela and López Gordillo, Yamila and Paradas Lara, Irene and Hernandes, Luzmarina and Catón Vázquez, Javier and Martínez Álvarez, Concepción (2014) Epidermal Growth Factor Impairs Palatal Shelf Adhesion and Fusion in the T gf-β3 Null Mutant. Cells Tissues Organs, 199 (2-3). pp. 201-2011. ISSN 1422-6405

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Official URL: http://dx.doi.org/10.1159/000362227




Abstract

The cleft palate presented by transforming growth factor-β3 (Tgf-β3 ) null mutant mice is caused by altered palatal shelf adhesion, cell proliferation, epithelial-to-mesenchymal transformation and cell death. The expression of epidermal growth factor (EGF), transforming growth factor-β1 ( Tgf-β1 ) and muscle segment homeobox-1 (Msx-1) is modified in the palates of these knockout mice, and the cell proliferation defect is caused by the change in EGF expression. In this study, we aimed to determine whether this change in EGF expression has any effect on the other mechanisms altered in Tgf-β 3 knockout mouse palates. We tested the effect of inhibiting
EGF activity in vitro in the knockout palates via the addition of Tyrphostin AG 1478. We also investigated possible interactions between EGF, Tgf-β 1 and Msx-1 in Tgf-β 3 null mouse palate cultures. The results show that the inhibition of EGF activity in Tgf-β 3 null mouse palate cultures improves palatal shelf adhesion and fusion, with a particular effect on cell death, and restores the normal distribution pattern of Msx-1 in the palatal esenchyme. Inhibition of TGF-β 1 does not affect either EGF or Msx-1 expression.


Item Type:Article
Additional Information:

Accepted after revision: March 14, 2014
Published online: May 23, 2014

Uncontrolled Keywords:Tgf-beta3 ; Tgf-beta1 ; Epidermal growth factor; Msx-1; Knockout; Palate development
Subjects:Medical sciences > Medicine
Medical sciences > Medicine > Physiology
Medical sciences > Medicine > Neurosciences
Medical sciences > Medicine > Dentistry
ID Code:38561
Deposited On:07 Sep 2016 12:58
Last Modified:19 Sep 2016 10:31

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