Publication: Molécula HLA-G y su importancia en la inmunorregulación de la unidad feto-materna. Aplicaciones en inmunoterapia celular
Loading...
Files
Official URL
Full text at PDC
Publication Date
2016-08-25
Authors
Advisors (or tutors)
Editors
Journal Title
Journal ISSN
Volume Title
Publisher
Universidad Complutense de Madrid
Citations
Exportar
Abstract
OBJETIVOS El objetivo principal de esta tesis doctoral consiste en determinar la presencia de la proteína HLA-G en la superficie celular de células madre CD34/CD133, células dendríticas mieloides y plasmacitoides, células dendríticas derivadas de células CD34/CD133 y derivadas de monocitos de sangre de cordón umbilical y sangre periférica materna, por técnicas de citometría de flujo y la expresión de la proteína HLA-G soluble en plasma de sangre de cordón umbilical por técnicas de ELISA. MATERIALES Y METODOS Se obtuvo un total de 35 unidades de sangre de cordón umbilical y 35 muestras de sangre periférica de gestantes a término que acudieron al Servicio de Ginecología y Obstetricia del Hospital Clínico Universitario San Carlos con cesáreas programadas, según aprobación de Comité Ético. Se realizaron técnicas de cultivo celular, citometría de flujo, Elisa y anticuerpos monoclonales, según protocolo, para la obtención de las células madre CD34+, células dendríticas mieloides y plasmacitoides del cordón umbilical y células dendríticas derivadas de células madre CD34+, y determinación de la molécula HLA-G, isoformas, nuevos alelos y polimorfismos...
OBJECTIVE The objective is to determine the presence of the HLA-G protein on the cell surface of stem cells CD34/CD133, myeloid and plasmacytoid dendritic cells, dendritic cells derived from CD34/CD133 and derived from blood monocytes cord and maternal peripheral blood by flow cytometry techniques and expression of soluble HLA-G protein in plasma from umbilical cord blood by ELISA. MATERIALS AND METHODS A total of 35 units of cord blood and peripheral blood samples from 35 pregnant women at term who attended Gynaecology and Obstetrics department, San Carlos Hospital with scheduled caesarean sections were made according Ethics Committee approval. Determining the molecule cell culture techniques performed, flow cytometry, ELISA and monoclonal antibodies, according to protocol, for obtaining CD34+ cells, myeloid dendritic cells and plasmacytoid cord and dendritic cells derived from CD34+ stem cells, and HLA-G isoforms, new alleles and polymorphism...
OBJECTIVE The objective is to determine the presence of the HLA-G protein on the cell surface of stem cells CD34/CD133, myeloid and plasmacytoid dendritic cells, dendritic cells derived from CD34/CD133 and derived from blood monocytes cord and maternal peripheral blood by flow cytometry techniques and expression of soluble HLA-G protein in plasma from umbilical cord blood by ELISA. MATERIALS AND METHODS A total of 35 units of cord blood and peripheral blood samples from 35 pregnant women at term who attended Gynaecology and Obstetrics department, San Carlos Hospital with scheduled caesarean sections were made according Ethics Committee approval. Determining the molecule cell culture techniques performed, flow cytometry, ELISA and monoclonal antibodies, according to protocol, for obtaining CD34+ cells, myeloid dendritic cells and plasmacytoid cord and dendritic cells derived from CD34+ stem cells, and HLA-G isoforms, new alleles and polymorphism...
Description
Tesis inédita de la Universidad Complutense de Madrid, Facultad de Medicina, Departamento de Obstetricia y Ginecología, leída el 01/07/2015