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Characterization of a new toxin from the entomopathogenic fungus Metarhizium anisopliae: the ribotoxin anisoplin

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Olombrada, Miriam and Medina, Pilar and Budia, Flor and Gavilanes, José G. and Martínez del Pozo, Álvaro and García Ortega, Lucía (2016) Characterization of a new toxin from the entomopathogenic fungus Metarhizium anisopliae: the ribotoxin anisoplin. Biological Chemistry . ISSN (Online) 1437-4315, ISSN (Print) 1431-6730 (Submitted)

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Official URL: http://www.degruyter.com/view/j/bchm.ahead-of-print/hsz-2016-0119/hsz-2016-0119.xml



Abstract

Metarhizium anisopliae is an entomopathogenic fungus relevant in biotechnology with applications like malaria vector control. Studies of its virulence factors are therefore of great interest. Fungal ribotoxins are toxic ribonucleases with extraordinary efficiency against target ribosomes and suggested as potential insecticides. Here, we describe this ribotoxin characteristic activity in M. anisopliae cultures. Anisoplin has been obtained as a recombinant protein and further characterized. It is structurally similar to hirsutellin A, the ribotoxin from the entomopathogen Hirsutella thompsonii. Moreover, anisoplin shows the ribonucleolytic activity typical of ribotoxins and cytotoxicity against insect cells. How Metarhizium uses this toxin and possible applications are on perspective.


Item Type:Article
Uncontrolled Keywords:hirsutellin; insecticide; ribonuclease; ribotoxin, sarcin.
Subjects:Sciences > Chemistry > Biochemistry
ID Code:39181
Deposited On:27 Sep 2016 10:28
Last Modified:02 Mar 2018 09:22

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