Universidad Complutense de Madrid
E-Prints Complutense

Salmonella Typhimurium exploits inflammation to its own advantage in piglets

Impacto

Downloads

Downloads per month over past year

Chirullo, Barbara and Pesciaroli, Michele and Drumo, Rosanna and Ruggeri, Jessica and Razzuoli, Elisabetta and Pistoia, Claudia and Petrucci, Paola and Martinelli, Nicola and Cucco, Lucilla and Moscati, Livia and Amadori, Massimo and Magistrali, Chiara F and Alborali, Giovanni L and Pasquali, Paolo (2015) Salmonella Typhimurium exploits inflammation to its own advantage in piglets. Frontiers in microbiology, 6 . p. 985. ISSN 1664-302X

[img]
Preview
PDF
Creative Commons Attribution.

1MB

Official URL: https://doi.org/10.3389/fmicb.2015.00985



Abstract

Salmonella Typhimurium (S. Typhimurium) is responsible for foodborne zoonotic infections that, in humans, induce self-limiting gastroenteritis. The aim of this study was to evaluate whether the wild-type strain S. Typhimurium (STM14028) is able to exploit inflammation fostering an active infection. Due to the similarity between human and porcine diseases induced by S. Typhimurium, we used piglets as a model for salmonellosis and gastrointestinal research. This study showed that STM14028 is able to efficiently colonize in vitro porcine mono-macrophages and intestinal columnar epithelial (IPEC-J2) cells, and that the colonization significantly increases with LPS pre-treatment. This increase was then reversed by inhibiting the LPS stimulation through LPS antagonist, confirming an active role of LPS stimulation in STM14028-intracellular colonization. Moreover, LPS in vivo treatment increased cytokines blood level and body temperature at 4 h post infection, which is consistent with an acute inflammatory stimulus, capable to influence the colonization of STM14028 in different organs and tissues. The present study proves for the first time that in acute enteric salmonellosis, S. Typhimurium exploits inflammation for its benefit in piglets.


Item Type:Article
Uncontrolled Keywords:Salmonella typhimurium, inflammation, immune response, pig, salmonellosis
Subjects:Medical sciences > Veterinary
ID Code:39616
Deposited On:19 Dec 2016 16:50
Last Modified:19 Dec 2016 16:50

Origin of downloads

Repository Staff Only: item control page