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Assessment of MALDI-TOF MS as Alternative Tool for Streptococcus suis Identification

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Pérez Sancho, Marta and Vela Alonso, Ana Isabel and García Seco, Teresa and Gottschalk, Marcelo and Domínguez Rodríguez, Lucas and Fernández Garayzábal, José Francisco (2015) Assessment of MALDI-TOF MS as Alternative Tool for Streptococcus suis Identification. Frontiers in Public Health, 3 . p. 202. ISSN 2296-2565

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Official URL: https://doi.org/10.3389/fpubh.2015.00202



Abstract

The accuracy of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) for identifying Streptococcus suis isolates obtained from pigs, wild animals, and humans was evaluated using a PCR-based identification assay as the gold standard. In addition, MALDI-TOF MS was compared with the commercial multi-tests Rapid ID 32 STREP system. From the 129 S. suis isolates included in the study and identified by the molecular method, only 31 isolates (24.03%) had score values ≥2.300 and 79 isolates (61.24%) gave score values between 2.299 and 2.000. After updating the currently available S. suis MALDI Biotyper database with the spectra of three additional clinical isolates of serotypes 2, 7, and 9, most isolates had statistically significant higher score values (mean score: 2.65) than those obtained using the original database (mean score: 2.182). Considering the results of the present study, we suggest using a less restrictive threshold score of ≥2.000 for reliable species identification of S. suis. According to this cut-off value, a total of 125 S. suis isolates (96.9%) were correctly identified using the updated database. These data indicate an excellent performance of MALDI-TOF MS for the identification of S. suis.


Item Type:Article
Uncontrolled Keywords:Identification, MALDI-TOF MS, Streptococcus suis, PCR, biochemical tests
Subjects:Medical sciences > Veterinary
ID Code:39618
Deposited On:20 Dec 2016 09:28
Last Modified:20 Dec 2016 09:28

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