Universidad Complutense de Madrid
E-Prints Complutense

Metagenomic detection of viral pathogens in Spanish honeybees: co-infection by Aphid Lethal Paralysis, Israel Acute Paralysis and Lake Sinai Viruses

Impacto

Downloads

Downloads per month over past year

Granberg, Fredrik and Vicente Rubiano, Marina and Rubio Guerri, Consuelo and Karlsson, Oskar E and Kukielka, Deborah and Belák, Sándor and Sánchez-Vizcaíno, José Manuel (2013) Metagenomic detection of viral pathogens in Spanish honeybees: co-infection by Aphid Lethal Paralysis, Israel Acute Paralysis and Lake Sinai Viruses. PLoS ONE, 8 (2). e57459. ISSN 1932-6203

[img]
Preview
PDF
Creative Commons Attribution.

615kB

Official URL: http://dx.doi.org/10.1371/journal.pone.0057459



Abstract

The situation in Europe concerning honeybees has in recent years become increasingly aggravated with steady decline in populations and/or catastrophic winter losses. This has largely been attributed to the occurrence of a variety of known and "unknown", emerging novel diseases. Previous studies have demonstrated that colonies often can harbour more than one pathogen, making identification of etiological agents with classical methods difficult. By employing an unbiased metagenomic approach, which allows the detection of both unexpected and previously unknown infectious agents, the detection of three viruses, Aphid Lethal Paralysis Virus (ALPV), Israel Acute Paralysis Virus (IAPV), and Lake Sinai Virus (LSV), in honeybees from Spain is reported in this article. The existence of a subgroup of ALPV with the ability to infect bees was only recently reported and this is the first identification of such a strain in Europe. Similarly, LSV appear to be a still unclassified group of viruses with unclear impact on colony health and these viruses have not previously been identified outside of the United States. Furthermore, our study also reveals that these bees carried a plant virus, Turnip Ringspot Virus (TuRSV), potentially serving as important vector organisms. Taken together, these results demonstrate the new possibilities opened up by high-throughput sequencing and metagenomic analysis to study emerging new diseases in domestic and wild animal populations, including honeybees.


Item Type:Article
Subjects:Medical sciences > Veterinary
ID Code:39659
Deposited On:21 Dec 2016 09:42
Last Modified:22 Dec 2016 11:55

Origin of downloads

Repository Staff Only: item control page