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Rapid identification and differentiation of Mycobacterium avium subspecies paratuberculosis types by use of real-time PCR and high-resolution melt analysis of the MAP1506 locus

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Castellanos, Elena and Aranaz Martín, Alicia and De Buck, Jeroen (2010) Rapid identification and differentiation of Mycobacterium avium subspecies paratuberculosis types by use of real-time PCR and high-resolution melt analysis of the MAP1506 locus. Journal of clinical microbiology, 48 (4). pp. 1474-7. ISSN 1098-660X

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Official URL: http://jcm.asm.org/content/48/4/1474.full



Abstract

High-resolution melt (HRM) analysis can identify sequence polymorphisms by comparing the melting curves of amplicons generated by real-time PCR amplification. We describe the application of this technique to identify Mycobacterium avium subspecies paratuberculosis types I, II, and III. The HRM approach was based on type-specific nucleotide sequences in MAP1506, a member of the PPE (proline-proline-glutamic acid) gene family.


Item Type:Article
Subjects:Medical sciences > Veterinary
ID Code:39691
Deposited On:10 Jan 2017 09:25
Last Modified:10 Jan 2017 09:25

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