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Characterization of the immune response induced by a commercially available inactivated bluetongue virus serotype 1 vaccine in sheep

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Pérez de Diego, Ana Cristina and Sánchez Cordón, Pedro José and Heras, Ana Isabel de las and Sánchez-Vizcaíno, José Manuel (2012) Characterization of the immune response induced by a commercially available inactivated bluetongue virus serotype 1 vaccine in sheep. TheScientificWorldJournal, 2012 . p. 147158. ISSN 1537-744X

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Official URL: http://dx.doi.org/10.1100/2012/147158



Abstract

The protective immune response generated by a commercial monovalent inactivated vaccine against bluetongue virus serotype 1 (BTV1) was studied. Five sheep were vaccinated, boost-vaccinated, and then challenged against BTV1 ALG/2006. RT-PCR did not detect viremia at any time during the experiment. Except a temperature increase observed after the initial and boost vaccinations, no clinical signs or lesions were observed. A specific and protective antibody response checked by ELISA was induced after vaccination and boost vaccination. This specific antibody response was associated with a significant increase in B lymphocytes confirmed by flow cytometry, while significant increases were not observed in T lymphocyte subpopulations (CD4(+), CD8(+), and WC1(+)), CD25(+) regulatory cells, or CD14(+) monocytes. After challenge with BTV1, the antibody response was much higher than during the boost vaccination period, and it was associated with a significant increase in B lymphocytes, CD14(+) monocytes, CD25(+) regulatory cells, and CD8(+) cytotoxic T lymphocytes.


Item Type:Article
Subjects:Medical sciences > Veterinary
ID Code:39702
Deposited On:21 Dec 2016 12:51
Last Modified:22 Dec 2016 11:58

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