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Auranofin-loaded nanoparticles as a new therapeutic tool to fight streptococcal infections

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Díez Martínez, Roberto y García Fernández, Esther y Manzano García, Miguel y Martínez, Ángel y Domenech, Mirian y Vallet Regí, María y García, Pedro (2016) Auranofin-loaded nanoparticles as a new therapeutic tool to fight streptococcal infections. Scientific Reports, 6 . ISSN 2045-2322

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URL Oficial: http://www.nature.com/



Resumen

Drug-loaded nanoparticles (NPs) can improve infection treatment by ensuring drug concentration at the right place within the therapeutic window. Poly(lactic-co-glycolic acid) (PLGA) NPs are able to enhance drug localization in target site and to sustainably release the entrapped molecule, reducing the secondary effects caused by systemic antibiotic administration. We have loaded auranofin, a gold compound traditionally used for treatment of rheumatoid arthritis,into PLGA NPs and their efficiency as antibacterial agent against two Gram-positive pathogens, Streptococcus pneumoniae and Streptococcus pyogenes was evaluated. Auranofin-PLGA NPs showed a strong bactericidal effect as cultures of multiresistant pneumococcal strains were practically sterilized after 6 h of treatment with such auranofin-NPs at 0.25 μM. Moreover, this potent bactericidal effect was also observed in S. pneumoniae and S. pyogenes biofilms, where the same concentration of auranofin-NPs was capable of decreasing the bacterial population about 4 logs more than free auranofin. These results were validated using zebrafish embryo model demonstrating that treatment with auranofin loaded into NPs achieved a noticeable survival against pneumococcal infections. All these approaches displayed a clear superiority of loaded auranofin PLGA nanocarriers compared to free administration of the drug, which supports their potential application for the treatment of streptococcal infections.


Tipo de documento:Artículo
Información Adicional:

RESEARCHER ID M-3378-2014 (María Vallet Regí)
ORCID 0000-0002-6104-4889 (María Vallet Regí)
RESEARCHER ID K-3719-2014 (Miguel Manzano García)
RESEARCHER ID M-3378-2014 (Miguel Manzano García)
ORCID 0000-0001-6238-6111 (Miguel Manzano García)

Supplementary information accompanies this paper at http://www.nature.com/srep
Competing financial interests: The authors declare no competing financial interests.
How to cite this article: Díez-Martínez, R. et al. Auranofin-loaded nanoparticles as a new therapeutic tool to
fight streptococcal infections. Sci. Rep. 6, 19525; doi: 10.1038/srep19525 (2016).

Palabras clave:Drug delivery, Antibiotics, Biofilms
Materias:Ciencias > Química
Ciencias > Química > Materiales
Ciencias > Química > Química inorgánica
Código ID:40811
Depositado:30 Ene 2017 17:05
Última Modificación:03 Feb 2017 12:23

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