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Auranofin-loaded nanoparticles as a new therapeutic tool to fight streptococcal infections

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Díez Martínez, Roberto and García Fernández, Esther and Manzano García, Miguel and Martínez, Ángel and Domenech, Mirian and Vallet Regí, María and García, Pedro (2016) Auranofin-loaded nanoparticles as a new therapeutic tool to fight streptococcal infections. Scientific Reports, 6 . ISSN 2045-2322

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Official URL: http://www.nature.com/



Abstract

Drug-loaded nanoparticles (NPs) can improve infection treatment by ensuring drug concentration at the right place within the therapeutic window. Poly(lactic-co-glycolic acid) (PLGA) NPs are able to enhance drug localization in target site and to sustainably release the entrapped molecule, reducing the secondary effects caused by systemic antibiotic administration. We have loaded auranofin, a gold compound traditionally used for treatment of rheumatoid arthritis,into PLGA NPs and their efficiency as antibacterial agent against two Gram-positive pathogens, Streptococcus pneumoniae and Streptococcus pyogenes was evaluated. Auranofin-PLGA NPs showed a strong bactericidal effect as cultures of multiresistant pneumococcal strains were practically sterilized after 6 h of treatment with such auranofin-NPs at 0.25 μM. Moreover, this potent bactericidal effect was also observed in S. pneumoniae and S. pyogenes biofilms, where the same concentration of auranofin-NPs was capable of decreasing the bacterial population about 4 logs more than free auranofin. These results were validated using zebrafish embryo model demonstrating that treatment with auranofin loaded into NPs achieved a noticeable survival against pneumococcal infections. All these approaches displayed a clear superiority of loaded auranofin PLGA nanocarriers compared to free administration of the drug, which supports their potential application for the treatment of streptococcal infections.


Item Type:Article
Additional Information:

RESEARCHER ID M-3378-2014 (María Vallet Regí)
ORCID 0000-0002-6104-4889 (María Vallet Regí)
RESEARCHER ID K-3719-2014 (Miguel Manzano García)
RESEARCHER ID M-3378-2014 (Miguel Manzano García)
ORCID 0000-0001-6238-6111 (Miguel Manzano García)

Supplementary information accompanies this paper at http://www.nature.com/srep
Competing financial interests: The authors declare no competing financial interests.
How to cite this article: Díez-Martínez, R. et al. Auranofin-loaded nanoparticles as a new therapeutic tool to
fight streptococcal infections. Sci. Rep. 6, 19525; doi: 10.1038/srep19525 (2016).

Uncontrolled Keywords:Drug delivery, Antibiotics, Biofilms
Subjects:Sciences > Chemistry
Sciences > Chemistry > Materials
Sciences > Chemistry > Chemistry, Inorganic
ID Code:40811
Deposited On:30 Jan 2017 17:05
Last Modified:03 Feb 2017 12:23

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