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3D scaffold with effective multidrug sequential release against bacteria biofilm

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García-Álvarez, Rafaela and Izquierdo-Barba, Isabel and Vallet Regí, María (2016) 3D scaffold with effective multidrug sequential release against bacteria biofilm. Acta Biomaterialia (49). pp. 113-126. ISSN ISSN: 1742-7061 (In Press)

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Abstract

Bone infection is a feared complication following surgery or trauma that remains as an extremely difficult disease to deal with. So far, the outcome of therapy could be improved with the design of 3D implants, which combine the merits of osseous regeneration and local multidrug therapy so as to avoid bacterial growth, drug resistance and the feared side effects. Herein, hierarchical 3D multidrug scaffolds based on nanocomposite bioceramic and polyvinyl alcohol (PVA) prepared by rapid prototyping with an external coating of gelatin-glutaraldehyde (Gel-Glu) have been fabricated. These 3D scaffolds contain three antimicrobial agents (rifampin, levofloxacin and vancomycin), which have been localized in different compartments of the scaffold to obtain different release kinetics and more effective combined therapy. Levofloxacin was loaded into the mesopores of nanocomposite bioceramic part, vancomycin was localized into PVA biopolymer part and rifampin was loaded in the external coating of Gel-Glu.
The obtained results show an early and fast release of rifampin followed by sustained and prolonged release of vancomycin and levofloxacin, respectively, which are mainly governed by the progressive in vitro degradability rate of these scaffolds. This combined therapy is able to destroy Gram-positive and Gram-negative bacteria biofilms as well as inhibit the bacteria growth; in addition, these multifunctional scaffolds exhibit excellent bioactivity as well as good biocompatibility with complete cell colonization of preosteoblast in the entire surface, ensuring good bone regeneration. These findings suggest that these hierarchical 3D multidrug scaffolds are promising candidates as platforms for local bone infection therapy.


Item Type:Article
Additional Information:

ORCID 0000-0002-6104-4889 (María Vallet Regí)
RESEARCHER ID M-3378-2014 (María Vallet Regí)

Uncontrolled Keywords:Multidrug 3D scaffold, combined therapy, biofilm, Gram-positive bacteria, Gran-negative bacteria and sequential antimicrobial delivery.
Subjects:Sciences > Chemistry > Biochemistry
Sciences > Chemistry > Materials
Sciences > Chemistry > Chemistry, Inorganic
ID Code:40847
Deposited On:24 Jan 2017 13:11
Last Modified:12 Nov 2018 00:01

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