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Nanocrystallinity effects on osteoblast and osteoclast response to silicon substituted hydroxyapatite



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Casarrubios, Laura y Matesanz Sancho, María Concepción y Sánchez Salcedo, Sandra y Arcos Navarrete, Daniel y Vallet Regí, María y Portoles, María Teresa (2016) Nanocrystallinity effects on osteoblast and osteoclast response to silicon substituted hydroxyapatite. Journal of Colloid and Interface Science, 482 . pp. 112-120. ISSN 0021-9797

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URL Oficial: https://www.elsevier.com/


Hypothesis: Silicon substituted hydroxyapatites (SiHA) are highly crystalline bioceramics treated at high temperatures (about 1200ºC) which have been approved for clinical use with spinal, orthopedic, periodontal, oral and craniomaxillofacial applications. The preparation of SiHA with lower temperature methods (about 700ºC) provides nanocrystalline SiHA (nano-SiHA) with enhanced bioreactivity due to higher surface area and smaller crystal size. The aim of this study has been to know the nanocrystallinity effects on the response of both osteoblasts and osteoclasts (the two main cell types involved in bone remodelling) to silicon substituted hydroxyapatite.
Experiments: Saos-2 osteoblasts and osteoclast-like cells (differentiated from RAW-264.7 macrophages)have been cultured on the surface of nano-SiHA and SiHA disks and different cell parameters have been evaluated: cell adhesion, proliferation, viability, intracellular content of reactive oxygen species, cell cycle phases, apoptosis, cell morphology, osteoclast-like cell differentiation and resorptive activity. Findings: This comparative in vitro study evidences that nanocrystallinity of SiHA affects the cell/biomaterial interface inducing bone cell apoptosis by loss of cell anchorage (anoikis), delaying osteoclast-like cell differentiation and decreasing the resorptive activity of this cell type. These results suggest the potential use of nano-SiHA biomaterial for preventing bone resorption in treatment of osteoporotic bone.

Tipo de documento:Artículo
Información Adicional:

RESEARCHER ID M-3378-2014 (María Vallet Regí)
ORCID 0000-0002-6104-4889 (María Vallet Regí)
RESEARCHER ID N-4501-2014 (Sandra Sánchez Salcedo)
ORCID 0000-0002-1889-2057 (Sandra Sánchez Salcedo)

Palabras clave:Nanocrystallinity; Hydroxyapatite; Silicon; Osteoclast; Osteoblast; Anoikis; Osteoporosis; Cell adhesion; Apoptosis; Cell cycle
Materias:Ciencias > Química > Materiales
Ciencias > Química > Química inorgánica
Código ID:40894
Depositado:03 Feb 2017 10:13
Última Modificación:16 Nov 2018 00:01

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