Universidad Complutense de Madrid
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Effects of nanocrystalline hydroxyapatites on macrophage polarization

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Linares, J. y Fernández, A. B. y Feito Castellano, María José y Matesanz Sancho, María Concepción y Sánchez-Salcedo, Sandra y Arcos Navarrete, Daniel y Vallet Regí, María y Rojo, J.M. y Portolés Pérez, M. T. (2016) Effects of nanocrystalline hydroxyapatites on macrophage polarization. Journal of Materials Chemistry B, 4 (11). pp. 1951-1959. ISSN 2050-750X

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Resumen

Silicon substituted and nanocrystalline hydroxyapatites have attracted the attention of many researchers due to their up-regulation in osteoblast cell metabolism and enhanced bioreactivity, respectively. On the other hand, the biomaterial success or failure depends ultimately on the immune response triggered after its implantation. Macrophages are the main components of the innate immune system with an important role in healing and tissue remodelling due to their remarkable functional plasticity, existing in a whole spectrum of functional populations with varying phenotypic features. The effects of nanocrystalline hydroxyapatite (nano-HA) and nanocrystalline silicon substituted hydroxyapatite (nano-SiHA) on the macrophage populations defined as pro-inflammatory (M1) and reparative (M2) phenotypes have been evaluated in the present study using RAW 264.7 cells and mouse peritoneal macrophages as in vitro models. M1 and M2 macrophage phenotypes were characterized by flow cytometry and confocal microscopy by the expression of CD80 and CD163, known as M1 and M2 markers, respectively. The polarization of primary macrophages towards the M1 or M2 phenotype was induced with the pro-inflammatory stimulus LPS or the anti-inflammatory stimulus IL-10, respectively, evaluating the biomaterial effects under these conditions. Our results show that both nano-HA and nano-SiHA favour the macrophage polarization towards an M2 reparative phenotype, decreasing M1 population and ensuring an appropriate response in the implantation site of these biomaterials designed for bone repair and bone tissue engineering.


Tipo de documento:Artículo
Información Adicional:

RESEARCHER ID M-3378-2014 (María Vallet Regí)
ORCID 0000-0002-6104-4889 (María Vallet Regí)
RESEARCHER ID N-4501-2014 (Sandra Sánchez Salcedo)
ORCID 0000-0002-1889-2057 (Sandra Sánchez Salcedo)

Palabras clave:Silicon-substituted hydroxyapatite, Signaling pathways, Activation, Bioceramics, Osteoblasts, Lymphocytes, Plasticity, Medicine, Behavior
Materias:Ciencias > Química > Materiales
Ciencias > Química > Química inorgánica
Código ID:40912
Depositado:09 Feb 2017 10:50
Última Modificación:03 Jul 2017 07:45

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