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Eph/ephrin-B-mediated cell-to-cell interactions govern MTS20+ thymic epithelial cell development

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Montero Herradon, Sara and Garcia-Ceca Hernández, Javier and Sánchez del Collado, Beatriz and Zapata González, Agustín (2016) Eph/ephrin-B-mediated cell-to-cell interactions govern MTS20+ thymic epithelial cell development. Histochemistry and cell biology, 146 (2). pp. 167-182. ISSN 0948-6143 (Print) 1432-119X (Online)

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Official URL: http://link.springer.com/article/10.1007%2Fs00418-016-1431-x



Abstract

Thymus development is a complex process in which cell-to-cell interactions between thymocytes and thymic epithelial cells (TECs) are essential to allow a proper maturation of both thymic cell components. Although signals that control thymocyte development are well known, mechanisms governing TEC maturation are poorly understood, especially those that regulate the maturation of immature TEC populations during early fetal thymus development. In this study, we show that EphB2-deficient, EphB2LacZ and EphB3-deficient fetal thymuses present a lower number of cells and delayed maturation of DN cell subsets compared to WT values. Moreover, deficits in the production of chemokines, known to be involved in the lymphoid seeding into the thymus, contribute in decreased proportions of intrathymic T cell progenitors (PIRA/B+) in the mutant thymuses from early stages of development. These features correlate with increased proportions of MTS20+ cells but fewer MTS20− cells from E13.5 onward in the deficient thymuses, suggesting a delayed development of the first epithelial cells. In addition, in vitro the lack of thymocytes or the blockade of Eph/ephrin-B-mediated cell-to-cell nteractions between either thymocytes–TECs or TECs–TECs in E13.5 fetal thymic lobes coursed with increased proportions of MTS20+ TECs. This confirms, for the first time, that the presence of CD45+ cells, corresponding at these stages to DN1 and DN2 cells, and Eph/ephrin-B-mediated heterotypic or homotypic cell interactions between thymocytes and TECs, or between TECs and themselves, contribute to the early maturation of MTS20+ TECs.


Item Type:Article
Uncontrolled Keywords:Eph/ephrins,Thymic epithelial cells, Thymus development, Thymocytes
Subjects:Medical sciences > Medicine > Immunology
Medical sciences > Biology > Cytology
ID Code:41131
Deposited On:06 Feb 2017 08:30
Last Modified:06 Feb 2017 15:27

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