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Electrochemical immunosensor for sensitive determination of transforming growth factor (TGF) - β1 in urine

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Sánchez Tirado, Esther and Martínez García, Gonzalo and González Cortés, Araceli and Yáñez Sedeño, Paloma and Pingarrón Carrazón, José Manuel (2017) Electrochemical immunosensor for sensitive determination of transforming growth factor (TGF) - β1 in urine. Biosensors and Bioelectronics, 88 . pp. 9-14. ISSN 0956-5663

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Official URL: https://doi.org/10.1016/j.bios.2016.05.093



Abstract

The first amperometric immunosensor for the quantification of TGF-β1, a cytokine proposed as a biomarker for patients having or at risk for renal disease, is described in this work. The immunosensor design involves disposable devices using carboxylic acid-functionalized magnetic microparticles supported onto screen-printed carbon electrodes and covalent immobilization of the specific antibody for TGF-β1 using Mix&Go polymer. A sandwich-type immunoassay was performed using biotin-anti-TGF and conjugation with peroxidase-labeled streptavidin (poly-HRP-Strept) polymer. Amperometric measurements were carried out at 0.20 V by adding hydrogen peroxide solution onto the electrode surface in the presence of hydroquinone as the redox mediator. The calibration plot allowed a range of linearity extending between 15 and 3000 pg/mL TGF-β1 which is adequate for the determination of the cytokine in plasma and urine. The limit of detection, 10 pg/mL, is notably improved with respect to those obtained with ELISA kits. The usefulness of the immunosensor for the determination of low TGF-β1 concentrations in real samples was evaluated by analyzing spiked urine at different pg/mL concentration levels.


Item Type:Article
Uncontrolled Keywords:Transforming growth factor-β1 (TGF-β1), Electrochemical immunosensor, Signal amplification, Urine
Subjects:Sciences > Chemistry
Sciences > Chemistry > Analytic chemistry
ID Code:41229
Deposited On:06 Sep 2017 10:35
Last Modified:05 Aug 2019 08:53

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